ivmМеждународный вестник ветеринарииInternational Journal of Veterinary Medicine2072-2419SpbGUVM Publishing House10.52419/issn2072-2419.2022.4.314ivm-1005Research ArticleБИОХИМИЯ, МОРФОЛОГИЯ, ФИЗИОЛОГИЯBIOCHEMISTRY, ANATOMY, PHYSIOLOGYВлияние полиморфизма гена FGF21 (g. 940 C/T) на биохимические показатели в сыворотке крови крупного рогатого скота голштинской породыThe effect of FGF21 gene polymorphism (g. 940C/T) on biochemical metabolic parameters in blood serum of holstein cattleСафинаН. Ю.SafinaN. Yu.

 к.б.н., с.н.с. 

 Candidate of Biological Sciences, Senior Researcher 

 Kazan 

ШакировШ. К.ShakrovSh. K.

 д.с.-х.н., проф., г.н.с. 

 Doctor of Agricultural Sciences, Professor, Chief Researcher 

 Kazan 

ГайнутдиноваЭ. Р.GaynutdinovaE. R.

 н.с.

 Researcher 

 Kazan 

ФаттаховаЗ. Ф.FattakhovaZ. F.

 к.б.н., с.н.с. 

 Candidate of Biological Sciences, Senior Researcher 

 Kazan 

Татарский научно-исследовательский институт сельского хозяйства ФИЦ КазНЦ РАНРоссияTatar Scientific Research Institute of Agriculture “Kazan Scientific Center of Russia Academy of Sciences”Russian Federation20220202202304314321Copyright © Сафина Н.Ю., Шакиров Ш.К., Гайнутдинова Э.Р., Фаттахова З.Ф., 20232023Сафина Н.Ю., Шакиров Ш.К., Гайнутдинова Э.Р., Фаттахова З.Ф.Safina N.Y., Shakrov S.K., Gaynutdinova E.R., Fattakhova Z.F.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vetjournal.spbguvm.ru/jour/article/view/1005

В исследовании рассмотрено влияние полиморфизма гена фактора роста фибробластов 21 (Bos taurus FGF21 - 940 C/T) на уровень FGF21 и биохимические показатели обмена веществ в сыворотке крови высокопродуктивных коров. Работа выполнялась в СХПК «ПЗ им. Ленина». Генотипирование ДНК 148 гол. коров голштинской породы проводили методом ПЦР-ПДРФ. В результате генетического типирования 148 гол. крупного рогатого скота идентифицированы два аллеля FGFC – 0,642 и FGFT – 0,358; и два генотипа FGFCC – 28,4% (42 гол.) и FGFTC – 71,6% (106 гол.). Особи с генотипом FGFTT не выявлены. Полученные данные демонстрируют, что у коров с генотипом FGF21CC уровень фермента FGF21 в сыворотке крови статистически значимо выше, чем у коров гетерозиготного генотипа на 165,5 пг/мл (28,2%; р < 0,001). Анализ взаимосвязи полиморфизма гена FGF21 и уровня фактора роста фибробластов 21 указывает на то, что в зависимости от генотипа изменяется активность этого фермента, вследствие чего наблюдается варьирование биохимических показателей сыворотки крови крупного рогатого скота голштинской породы.

The study examined the effect of polymorphism of the fibroblast growth factor 21 gene (Bos taurus FGF21 g.940 C/T) on the level of FGF21 and biochemical metabolic parameters in blood serum of highproducing cows. DNA genotyping of 148 animals of Holstein cows was performed by a PCR-RFLP method. As a result of genotyping, two FGFC – 0.642 and FGFT – 0.358 alleles as well as two FGFCC – 28.4% (42 animals) and FGFTC – 71.6% (106 animals) genotypes were identified. Individuals with the FGFTT genotype were not identified. The obtained data show that the seroen zyme FGF21 level is statistically significantly higher in cows with the FGF21CC genotype than in cows of the heterozygous genotype by 165.5 pg/ml (28.2%; p < 0.001). Analysis of the association between the polymorphism of the FGF21 gene and the level of fibroblast growth factor 21 suggests that the activity of this enzyme changes depending on the genotype, as a result of which there is a variation in serum biochemical parameters of Holstein cattle.

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The authors declare that there are no conflicts of interest present.