ivmМеждународный вестник ветеринарииInternational Journal of Veterinary Medicine2072-2419SpbGUVM Publishing House10.52419/issn2072-2419.2023.1.11ivm-1052Research ArticleИНФЕКЦИОННЫЕ БОЛЕЗНИINFECTIOUS DISEASESСравнительная оценка биологической активности комплекса ультрадисперсных частиц на основе меди и цинка в тесте ингибирования биолюминесценцииComparative evaluation of the biological activity of a complex of ultrafine particles based on copper and zinc in the bioluminescence inhibition testhttps://orcid.org/0000-0002-5125-5981СизоваЕ. А.SizovaE. A.

д. б.н., вед. науч. сотр

Doctor of Biological Sciences, Leading Researcher

https://orcid.org/0000-0002-1589-2211ЯушеваЕ. В.YaushevaE. V.

к.б.н., ст. науч. сотр.

PhD of Biological Sciences, Senior Researcher

https://orcid.org/0000-0002-8755-414XНечитайлоК. С.NechitailoK. S.

науч. сотр.

ФГБНУ «Федеральный научный центр биологических систем и агротехнологий Российской академии наук»РоссияFederal Research Centre of Biological Systems and Agro-technologies of the Russian Academy of SciencesRussian Federation202315062023011119Copyright © Сизова Е.А., Яушева Е.В., Нечитайло К.С., 20232023Сизова Е.А., Яушева Е.В., Нечитайло К.С.Sizova E.A., Yausheva E.V., Nechitailo K.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vetjournal.spbguvm.ru/jour/article/view/1052

Современные методы нанотехнологии позволяют преобразовывать макро- и микрообъекты в ультрадисперсные частицы (УДЧ), при этом сам процесс сопровождается изменением химических и физических свойств основного материала. Закономерно, биоактивность комплексов УДЧ микроэлементов будет отличаться от таковой у отдельных УДЧ. В связи с этим, цель нашего исследования состояла в изучении зависимости биологической активности комплекса УДЧ меди и цинка от их физико-химических характеристик. В эксперименте были использованы три образца УДЧ медь + цинк (A, B, C), полученные путем газофазного синтеза.Анализ полученных данных позволил констатировать, что высоким уровнем токсичности исследуемых образцов УДЧ в отношении штамма E.coli E. coli K12 TG1 pF1 обладал образец «С», процент фазы металлического цинка, в котором, в сравнении с другими тестируемыми порошками, наибольший, а процент фазы металлической меди наименьший.В результате, данные на основе реакции штаммов E.coli pSoxS::lux и E. coli pKatGlux, индукция свечения которых связана с развитием окислительного стресса в клетках при действии исследуемых образцов комплекса медь+цинк позволила выявить образец с максимальным эффектом – «С». Далее по степени развития токсического эффекта в ряду убывали образцы «B» и «A». В тоже время, интерпретация результатов отклика штамма E. coli pRecA-lux, формировало иной ряд специфичности: наибольший эффект на клетки бактерий среди исследуемых веществ оказывал образец «B».Исходя из полученных данных, в нашем исследовании степень биологической активности УДЧ для живой системы определялась совокупностью ряда физико-химических параметров: форма, фазовый состав и удельная поверхность. Так, высокий уровень токсичности коррелировал с наибольшим процентом фазы металлического цинка, наименьшим процентом металлической меди и оксидного слоя цинка в сочетанием с наименьшей удельной поверхностью.

Modern methods of nanotechnology make it possible to transform macro- and micro-objects into ultrafine particles (UFP), while the process itself is accompanied by a change in the chemical and physical properties of the base material. Naturally, the biological activity of complexes of UHF microelements will differ from that of individual UHFs. In this regard, the purpose of our study was to study the dependence of the biological activity of the UHF copper + zinc complex on their physicochemical characteristics.Analysis of the data obtained allowed us to state that sample «C» had a high level of toxicity of the studied samples of UHF against the strain E. coli E. coli K12 TG1 pF1. Further, according to the observed effect, sample A was noted, while the effect of exposure to the studied UHF was dose- and time-dependent.As a result, the data obtained on the basis of the reaction of strains E. coli pSoxS::lux and E. coli pKatG-lux, the induction of luminescence of which is associated with the development of oxidative stress in cells, on the action of the studied samples of the copper+ zinc complex, made it possible to form a sample with the maximum effect «C». Further, according to the degree of development of the toxic effect, samples «B» and «A» decreased in the series. At the same time, the interpretation of the results of the response of the E. coli pRecA -lux strain, the induction of which is caused by DNA damage in cells, forms a different series of specificity: the greatest effect on bacterial cells among the studied substances, sample B was rendered, while the maximum luminescence amplitude was 70 units at 0.002 M, and the sensitivity threshold was at a concentration of 0.0004 M.Based on the data obtained, in our study, the degree of biological activity of UHF for a living system was determined by a combination of a number of physicochemical parameters: shape, phase composition, and specific surface area

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The authors declare that there are no conflicts of interest present.