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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ivm</journal-id><journal-title-group><journal-title xml:lang="ru">Международный вестник ветеринарии</journal-title><trans-title-group xml:lang="en"><trans-title>International Journal of Veterinary Medicine</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-2419</issn><publisher><publisher-name>SpbGUVM Publishing House</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.52419/issn2072-2419.2023.2.194</article-id><article-id custom-type="elpub" pub-id-type="custom">ivm-1242</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ЗООГИГИЕНА, САНИТАРИЯ, КОРМЛЕНИЕ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>ZOOHYGIENE, SANITATION, FEEDING</subject></subj-group></article-categories><title-group><article-title>Обнаружение ДНК бактерий рода Helicobacter в мясе свиней</article-title><trans-title-group xml:lang="en"><trans-title>Detection of DNA of bacteria of the genus Helicobacter in pig meat</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6855-3387</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Поздеев</surname><given-names>О. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Pozdeev</surname><given-names>O. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>заведующий кафедрой микробиологии, доктор медицинских наук, профессор</p></bio><bio xml:lang="en"><p>Head of the Department of Microbiology, professor </p></bio><email xlink:type="simple">nurgalievfm@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7496-0379</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Нургалиев</surname><given-names>Ф. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Nurgaliev</surname><given-names>F. M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доцент кафедры микробиологии, вирусологии и иммунологии, кандидат ветеринарных наук</p></bio><bio xml:lang="en"><p>Associate Professor of the Department of Microbiology, Virology and  immunology </p></bio><email xlink:type="simple">nurgalievfm@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7053-6925</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гильманов</surname><given-names>Х. Х.</given-names></name><name name-style="western" xml:lang="en"><surname>Gilmanov</surname><given-names>Kh. Kh.</given-names></name></name-alternatives><bio xml:lang="ru"><p>научный консультант, кандидат биологических наук </p></bio><bio xml:lang="en"><p>scientific consultant </p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Маннанова</surname><given-names>А. Р.</given-names></name><name name-style="western" xml:lang="en"><surname>Mannanova</surname><given-names>A. R.</given-names></name></name-alternatives><bio xml:lang="ru"><p>студент</p></bio><bio xml:lang="en"><p>student</p></bio><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>КГМА - филиал ФГБОУ ДПО РМАНПО Минздрава России</institution><country>Россия</country></aff><aff xml:lang="en"><institution>KSMA - Branch Campus of the FSBEIFPE RMACPE MOH Russia</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФГБОУ ВО Казанская ГАВМ, кандидат ветеринарных наук</institution><country>Россия</country></aff><aff xml:lang="en"><institution>FSBEI HE Kazan SAVM</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>ФГБНУ ФНЦ ВИЭВ РАН</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal State Budget Scientific Institution "Federal Scientific Centre VIEV"</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>ФГБОУ ВО Казанская ГАВМ</institution><country>Россия</country></aff><aff xml:lang="en"><institution>FSBEI HE Kazan SAVM</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2023</year></pub-date><pub-date pub-type="epub"><day>29</day><month>06</month><year>2023</year></pub-date><volume>0</volume><issue>2</issue><fpage>194</fpage><lpage>205</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Поздеев О.К., Нургалиев Ф.М., Гильманов Х.Х., Маннанова А.Р., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Поздеев О.К., Нургалиев Ф.М., Гильманов Х.Х., Маннанова А.Р.</copyright-holder><copyright-holder xml:lang="en">Pozdeev O.K., Nurgaliev F.M., Gilmanov K.K., Mannanova A.R.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vetjournal.spbguvm.ru/jour/article/view/1242">https://vetjournal.spbguvm.ru/jour/article/view/1242</self-uri><abstract><p>Хеликобактеры, отличные от H. pylori, колонизирующие различных животных, также способны вызывать гастриты, язвы желудка и MALT-лимфомы у человека. Среди подобных бактерий со слизистой оболочки желудка человека наиболее часто выделяют H. suis. Несмотря на свое название, этот микроорганизм может также инфицировать кошек и собак. При этом распространенность H. Suis среди поголовья свиней в различных странах варьирует в пределах 10,8-90,0%, но в большинстве исследований она составляет, в среднем, 60%. Очевидно, что такое масштабное распространение H. suis и тесные контакты с инфицированными животными могут способствовать профессиональным заражениям людей. Кроме того, потребление зараженного и недостаточно термически обработанного свиного мяса является возможным путем передачи этого микроорганизма. В наших экспериментах исследованием на наличие ДНК H. suis в различных частях туш свиней непосредственно после убоя установлено, что в 26,7 % пробах ДНК бактерий была обнаружена на слизистой оболочке ротовой полости, 13,3 % – по линиям разреза шеи, 6,6 % – грудного отдела и 0 % – окорока. Поскольку, по данным доступной литературы, выделение H. suis бактериологическим методом представляет большие трудности, то одним из немногих доступных способов диагностики остается обнаружение нуклеиновых кислот микроорганизма. Для обнаружения ДНК жизнеспособных H. suis в мясе свиней мы использовали протокол обработки образцов свинины этидия моноазидом в сочетании с ПЦР для исследования мяса свиней (свиная шейка) различных производителей, приобретенных на прилавках рынков г. Казани. В результате, в 0,5 % проб была обнаружена ДНК живых H. suis.</p></abstract><trans-abstract xml:lang="en"><p>Helicobacters different from Helicobacter pylori, colonizing various animals, are also capable of causing gastritis, stomach ulcers and MALT – lymphomas in humans. Helicobacter suis is most often isolated among similar bacteria from the mucous membrane of the human stomach. In spite of its name this microorganism can also infect cats and dogs. At the same time the spread of Helicobacter suis among the pig population in different countries varies between 10.8-90.0% but in most studies it is in average 60%. It is obvious that such extensive spread of Helicobacter suis and close contacts with infected animals can contribute to occupational infections of human beings. In addition, the consumption of infected and insufficiently heat-treated pork meat is a possible route of transmission of this microorganism. In our studies on the presence of Helicobacter suis DNA in various parts of pig carcasses immediately after slaughter determined that DNA samples in 26.7% of bacterial were found on the oral mucosa, 13.3% – along the neck lines section, 6.6% - thoracic section and 0% – hock section. According to the available literature the isolation of Helicobacter suis by the bacteriological method presents great difficulties and the detection of nucleic acids of the microorganism remains as one of the few available diagnostic methods. To detect the DNA of living Helicobacter suis in pork we used the protocol for processing pork samples with ethidium monoazide in combination with PCR to study pig meat (pork neck) from various manufacturers purchased on the shelves of Kazan markets. As a result, the DNA of living Helicobacter suis was found in 0.5% of the samples.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Helicobacter suis</kwd><kwd>гастриты</kwd><kwd>лабораторная диагностика</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Helicobacter suis</kwd><kwd>gastritis</kwd><kwd>laboratory diagnostics</kwd><kwd>pork</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Исаева Г. Ш., Исаева Р. А. Геном Helicobacter pylori и патогенность. Бактериология. 2021;6(1):37-47. DOI: 10.20953/2500-1027-2021-1-37-47. 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