ivmМеждународный вестник ветеринарииInternational Journal of Veterinary Medicine2072-2419SpbGUVM Publishing House10.52419/issn2072-2419.2024.2.268ivm-1398Research ArticleАКУШЕРСТВО, ГИНЕКОЛОГИЯOBSTETRICS, GYNECOLOGYПрименение сурфагона для индукции овуляции кобыл в циклах ВРТThe use of surfagon for the induction of ovulation of mares in ART cycleshttps://orcid.org/0000-0001-9845-1691КалашниковВ. В.KalashnikovV. V.

акад. РАН, д-р с.-х. наук, проф., науч. рук. ФГБНУ «ВНИИконеводства» 

acad. RAS, Doctor of Agricultural Sciences, prof., Scientific director    

https://orcid.org/0000-0001-6960-5233ЛебедеваЛ. Ф.LebedevaL. F.

д-р с.-х. наук, доц., зав. лаб. физиологии

Doctor. Agricultural Sciences, Associate Professor, Head. Of the lab. Physiology  

Lebedeva-L18@yandex.ruhttps://orcid.org/0000-0003-3495-3478СолодоваЕ. В.SolodovaE. V.

канд. биол. наук, ст. науч. сотр.

Candidate of Biol. Sciences, scientific co-workers 

КрасильниковаЕ. А.KrasilnikovaE. A.

 вет. врач 

 veterinarian 

ФГБНУ «Всероссийский научно-исследовательский институт коневодства»РоссияFederal State Institute "The All-Russian Research Institute for Horse Breeding"Russian FederationООО «Поворот ВП»Россия«Povorot» VP LLCRussian Federation20242806202402268275Copyright © Калашников В.В., Лебедева Л.Ф., Солодова Е.В., Красильникова Е.А., 20242024Калашников В.В., Лебедева Л.Ф., Солодова Е.В., Красильникова Е.А.Kalashnikov V.V., Lebedeva L.F., Solodova E.V., Krasilnikova E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vetjournal.spbguvm.ru/jour/article/view/1398

С целью прогнозирования сроков овуляции кобыл для получения зрелых ооцитов и фолликулярной жидкости в циклах вспомогательных репродуктивных технологий (ВРТ) применяли отечественный аналог ГнРГ сурфагон в двух дозах (40 и 50 мкг) на фоне сравнения с эффективностью ранее применяемого препарата ХГЧ. Инъекции сурфагона проводили внутримышечно при достижении лидирующим фолликулом диаметра 35 мм и более, наличии максимального или начавшего снижаться отёка матки. Анализ продолжительности интервалов между внутримышечной инъекцией 40 мкг и 50 мкг препарата и овуляцией показал, что в промежутке между 37 и 48 часами после инъекции происходит 72,4 и 35% овуляций, соответственною. Результат применения 40 мкг сурфагона аналогичен результатам применения ХГЧ - 72 и 74% овуляций в данном промежутке, соответственно. Следовательно, препарат «Сурфагон» в дозе 40 мкг наиболее приемлем для применения в циклах ВРТ. Всего 3,4% овуляций после применения 40 мкг сурфагона происходит в интервале до 24 часов после инъекции. Поэтому его применение позволяет планировать забор фолликулярной жидкости и ооцита из преовуляторного фолликула методом OPU через 24 часа. Отечественный препарат Сурфагон (люлеберин ацетат, ЗАО «Мосагроген», Москва) эффективен для индукции овуляции у кобыл и может служить альтернативой использованию ХГЧ и импортных аналогов ГнРГ как в обычной практике воспроизводства, так и в планировании процедур OPU с целью забора фолликулярной жидкости и извлечения ооцитов.

In order to predict the timing of ovulation in mares, to obtain mature oocytes and follicular fluid in ART cycles, the domestic analogue of GnRH surfagon was used in two doses (40 and 50 mcg) against the background of comparison with the effectiveness of the previously used hCG drug. Surfagon injections were carried out intramuscularly when the leading follicle reached a diameter of 35 mm or more, and there was maximum or decreased edema of the uterus. An analysis of the duration of the intervals between intramuscular injection of 40 µg and 50 µg of the drug and ovulation showed that 72.4 and 35% of ovulations occur, respectively, between 37 and 48 hours after injection. The result of using 40 µg of surfagon is similar to the results of using hCG - 72 and 74% of ovulations in this period, respectively. Therefore, the drug “Surfagon” at a dose of 40 µg is most suitable for use in ART cycles. Only 3.4% of ovulations after the use of 40 µg of surfagon occur in the interval up to 24 hours after injection. Therefore, its use allows planning the collection of follicular fluid and oocyte from the preovulatory follicle using the OPU method after 24 hours. The domestic drug Surfagon (lyuleberin acetate, CJSC Mosagrogen, Moscow) is effective for the induction of ovulation in mares and can serve as an alternative to the use of HCG and imported GnRH analogues both in normal reproduction practice and in planning OPU procedures for the purpose of follicular fluid intake and oocyte extraction.

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The authors declare that there are no conflicts of interest present.