ivmМеждународный вестник ветеринарииInternational Journal of Veterinary Medicine2072-2419SpbGUVM Publishing House10.52419/issn2072-2419.2024.2.285ivm-1400Research ArticleАКУШЕРСТВО, ГИНЕКОЛОГИЯOBSTETRICS, GYNECOLOGYПрогностическая оценка качества ооцитов у кобыл, использующихся в программах ВРТ, по биохимическим показателям фолликулярной жидкости и сыворотки кровиPrognostic assessment of the quality of oocytes in mares used in ART programs based on biochemical parameters of follicular fluid and blood serumhttps://orcid.org/0000-0001-9845-1691КалашниковВ. В.KalashnikovV. V.

 акад. РАН, д-р с.-х. наук, проф., науч. рук. 

acad. RAS, Doctor of Agricultural Sciences, prof., Scientific director 

https://orcid.org/0000-0001-6960-5233ЛебедеваЛ. Ф.LebedevaL. F.

д-р с.-х. наук, доц., зав. лаб. физиологии

Doctor. Agricultural Sciences, Associate Professor, Head of Physiology lab. 

Lebedeva-L18@yandex.ruhttps://orcid.org/0000-0003-3495-3478СолодоваЕ. В.SolodovaE. V.

 канд. биол. наук, ст. науч. сотр. 

Candidate of Biol. Sciences, scientific co-workers 

https://orcid.org/0000-0002-8102-4463БаковецкаяО. В.BakovetskayaO. V.

д-р биол. наук. проф., зав. каф. биологии

Doctor of Biology. Sci. prof., Head of Biology department  

ФГБНУ «Всероссийский научно-исследовательский институт коневодства»РоссияThe All-Russian Research Institute for Horse breedingRussian FederationФГБОУ ВО Рязанский государственный медицинский университет имени академика И.П. ПавловаРоссияRyazan State Medical University named after Academician I.P. PavlovRussian Federation20242806202402285297Copyright © Калашников В.В., Лебедева Л.Ф., Солодова Е.В., Баковецкая О.В., 20242024Калашников В.В., Лебедева Л.Ф., Солодова Е.В., Баковецкая О.В.Kalashnikov V.V., Lebedeva L.F., Solodova E.V., Bakovetskaya O.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vetjournal.spbguvm.ru/jour/article/view/1400

Исследован биохимический состав жидкости, содержащейся в фолликулах кобыл, а также установлен уровень идентичности данного состава биохимическим показателям их крови. В результате исследований показано, что в фолликулярной жидкости (ФЖ) крупных фолликулов (≥37 мм) содержится значительно больше электролитов - Na, К, P и сывороточного железа, но существенно меньше общего белка, мочевой кислоты, холестерина, триглицеридов, ХС ЛПВП и внутриклеточных ферментов - АЛТ, АСТ, КФК, ЛДГ, ГГТП, ЩФ, чем в сыворотке крови (p<0,05). При этом не найдено достоверных различий по содержанию в исследованных средах глюкозы, мочевины, магния, альбумина, всех видов билирубина, креатинина, ХС ЛПНП, альфа-амилазы, кальция. Показано наличие корреляционных связей между количественными показателями большинства компонентов биохимического состава ФЖ и сыворотки крови, а в самой ФЖ - между компонентами липидного профиля с кальцием, фосфором, глюкозой, альбумином. Значимые связи установлены также между содержанием в ФЖ и сыворотке крови сывороточного Fe, Mg, Ca, общего белка, мочевины, холестерина, триглицеридов, внутриклеточных ферментов - АЛТ, АСТ, КФК, ЛДГ, ГГТП, ЩФ, билирубина и креатинина (p<0,05). Полученные статистически достоверные экспериментальные данные могут быть использованы для прогностической оценки состава фолликулярной жидкости и соответствующего качества ооцитов по биохимическому анализу сыворотки крови кобыл. Полученные статистически достоверные экспериментальные данные могут быть использованы для прогностической оценки состава фолликулярной жидкости и соответствующего качества ооцитов по биохимическому анализу сыворотки крови кобыл.

The biochemical composition of the mare’s follicle fluid was studied, and the level of identity of this composition with the biochemical parameters of their blood was established. It has been shown that the follicular fluid (FF) of large follicles (≥37 mm) contains significantly more electrolytes – Na, K, P and serum Fe, but significantly less total protein, uric acid, cholesterol, triglycerides, HDL cholesterol and intracellular enzymes - ALT, AST, CPK, LDH, GGTP, alkaline phosphatase, than in blood serum (p<0.05). At the same time, no significant differences were found in the content of glucose, urea, magnesium, albumin, all types of bilirubin, creatinine, LDL cholesterol, alphaamylase, and calcium in the studied media. The presence of correlations has been shown between the quantitative indicators of most components of the biochemical composition of the FF and blood serum, and in the FF itself - between the components of the lipid profile with Ca, P, glucose, and albumin. Significant relationships were also established between the content of serum Fe, Mg, Ca, total protein, urea, cholesterol, triglycerides, intracellular enzymes - ALT, AST, CPK, LDH, GGTP, alkaline phosphatase, bilirubin and creatinine in the FF and blood serum (p<0. 05). The obtained statistically reliable experimental data can be used for prognostic assessment of the composition of follicular fluid and the corresponding quality of oocytes based on biochemical analysis of blood serum in mares. The statistically reliable experimental data obtained can be used for a prognostic assessment of the composition of follicular fluid and the corresponding quality of oocytes by biochemical analysis of blood serum of mares.

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The authors declare that there are no conflicts of interest present.