ivmМеждународный вестник ветеринарииInternational Journal of Veterinary Medicine2072-2419SpbGUVM Publishing House10.52419/issn2072-2419.2024.3.402ivm-1486Research ArticleАКУШЕРСТВО, ГИНЕКОЛОГИЯOBSTETRICS, GYNECOLOGYАссоциация однонуклеотидных полиморфизмов генов FASN И DGAT1 с живой массой у мясного скотаAssociation of single nucleotide polymorphisms of FASN and DGAT1 genes with live weight in beef cattlehttps://orcid.org/0000-0002-9164-4199ШевхужевА. Ф.ShevkhuzhevA. F.

Шевхужев А.Ф. – д-р с.-х. наук, проф.

Shevkhuzhev A.F. – Professor, Doctor of Agricultural Sciences

https://orcid.org/0000-0002-6090-4453СкорыхЛ. Н.SkorykhL. N.

Скорых Л.Н. – д-р биол. наук, доц.

Skorykh L.N. – Associate Professor, Doctor of Biological Sciences

https://orcid.org/0000-0003-0130-3639КриворучкоА. Ю.KrivoruchkoA. Yu.

Криворучко А.Ю. – д-р биол. наук 

Krivoruchko A.Yu. – Doctor of Biological Sciences

https://orcid.org/0000-0002-2193-7498СкоковаА. В.SkokovaA. V.

Скокова А.В. – канд. биол. наук 

Skokova A.V. – cand. Biol. sciences

https://orcid.org/0000-0003-3003-4175КаниболоцкаяА. А.KaniolotskayaA. A.

Каниболоцкая А.А. – канд. биол. наук 

Kaniolotskaya A.A. – PhD. Biol. sciences

ФГБНУ «Северо-Кавказский ФНАЦ»РоссияFSBSI "North Caucasian FSAC"Russian Federation20242712202403402412Copyright © Шевхужев А.Ф., Скорых Л.Н., Криворучко А.Ю., Скокова А.В., Каниболоцкая А.А., 20242024Шевхужев А.Ф., Скорых Л.Н., Криворучко А.Ю., Скокова А.В., Каниболоцкая А.А.Shevkhuzhev A.F., Skorykh L.N., Krivoruchko A.Y., Skokova A.V., Kaniolotskaya A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vetjournal.spbguvm.ru/jour/article/view/1486

Отечественная калмыцкая порода скота считается уникальной и отличается от всех разводимых на территории России мясных пород. Животные этой породы наделены природной выносливостью и независимостью от климатических условий содержания, отличаются высокой адаптационной способностью, что дает возможность для разведения их как в чистоте, так в различных схемах скрещивания с другими породами во многих областях и регионах нашей страны. Кроме того, мясо от животных этой породы имеет достаточно высокие качественные и вкусовые показателями. Учитывая возрастающую потребность в высокачественной говядине, необходимость в исследованиях, направленных на повышение мясной продуктивности крупного рогатого скота, является актуальной задачей сельскохозяйственной науки и практики. Цель работы заключается в изучении полиморфизмов генов FASN, DGAT1 и анализе их взаимосвязи с живой массой в популяции бычков калмыцкой породы, разводимых в крайне засушливой климатической и полупустынной эколого-ландшафтной зоне Ставропольского края. Исследования проводили в условиях СПК (колхоз-племзавод) «Дружба» Ставропольского края на бычках калмыцкой породы (n=156) в возрасте 8 месяцев. Для изучения полиморфизмов генов FASN и DGAT1 выполнили генотипирование методом ПЦР-ПДРФ c использованием лиофилизованных готовых реакционных смесей GenPak® PCR Core и пар праймеров, подобранных на ресурсе Primer-BLAST. Установили, что животные носители AG и GG генотипов полиморфизма g.17924A> G гена FASN имели живую массу на 7,8 и 11,9 % больше по сравнению с АА генотипом, а также среди всего исследованного поголовья по двум изучаемым генам. Животные носители гомозиготного ТТ генотипа полиморфизма c.1416T> G в гене DGAT1 имели более высокую живой массой среди всех исследованных животных по этому гену на 5 %. 

The domestic Kalmyk cattle breed is considered unique and differs from all meat breeds bred in Russia. Animals of this breed are endowed with natural endurance and independence from climatic conditions, they are characterized by high adaptive ability, which makes it possible to breed them both in purity and in various schemes of crossing with other breeds in many regions and regions of our country. In addition, beef from animals of this breed has quite high quality and taste indicators. Given the increasing need for high-quality beef, the need for research aimed at increasing the meat productivity of cattle is an urgent task of agricultural science and practice.The aim of the work is to study the polymorphisms of the FASN and DGAT1 genes and their association with live weight in a population of Kalmyk bull calves bred in an extremely arid climatic and semi-arid ecological landscape zone of the Stavropol Territory. The studies were conducted in the conditions of SPK (kolkhozplemzavod) "Druzhba" (Druzhba collective farm) of Stavropol Krai on Kalmyk steers (n=156) at the age of 8 months. To study the polymorphisms of the FASN and DGAT1 genes, genotyping was performed by PCRPDRF using lyophilized ready-made GenPak ® PCR Core reaction mixtures using a pair of primers selected on the Primer-BLAST resource. It was found that animals carrying AG and GG genotypes in the replacement of g.17924A>G of the FASN gene had a live weight of 7.8 and 11.9% more than the AA genotype, as well as among the entire studied livestock for the two studied genes. Animals carrying the homozygous TT genotype of c.1416T > G polymorphism in the DGAT1 gene had a higher live weight among all animals studied for this gene by 5%.

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The authors declare that there are no conflicts of interest present.