The studies were conducted to determine the minimum inhibitory concentration of ciprofloxacin in relation to various species of salmonella and to study the therapeutic and prophylactic efficacy of civetin (an antimicrobial drug based on ciprofloxacin) in experimental salmonellosis of the Haysex Brown cross chickens in the course of the work. Determination of the minimum inhibitory concentration of ciprofloxacin for Salmonella enteritidis, Salmonella typhimurium and Salmonella infantis was performed by double serial dilution in a liquid nutrient medium and using HiComb Strip test (HiMedia Laboratories Pvt. Limited, In-dia).The therapeutic and prophylactic efficacy of ciprofloxacin in experimental salmonellosis of chickens of different etiology was studied on 650 males that were divided into 13 groups. Experimental infections were reproduced by three species of Salmonella: Salmonella enteritidis, Salmonella typhimurium and Salmonella infantis. The microorganisms were injected into the chicks intraperitoneally at a concentration of 300 million CFU / 0.5 ml. Ciprofloxacin was administered in a freely available form with drinking water in concentrations of 50, 100 and 200 mg/l of water.The minimum inhibitory concentration of ciprofloxacin for Salmonella enteritidis was 0.25 pg / ml, for Salmonella typhimurium 0.001-0.004 pg/ml, for Salmonella infantis 0.01 pg/ml.It was found that oral administration (freely available with drinking water) of civetin for five days in concentrations of 50-100 mg/l of water to chicks experimentally infected with Salmonella enteritidis failed to achieve a positive therapeutic and prophylactic ef-fect.Effective (82%) was the appointment of ciprofloxacin in a concentration of 200 mg/l water. Among the chickens infected with Salmonella typhimurium and Salmonella infantis effective (96-100%) was the appointment of ciprofloxacin in all doses (50200 mg/l). It should be noted that in the control groups only 64% and 62% of chickens died.