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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ivm</journal-id><journal-title-group><journal-title xml:lang="ru">Международный вестник ветеринарии</journal-title><trans-title-group xml:lang="en"><trans-title>International Journal of Veterinary Medicine</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-2419</issn><publisher><publisher-name>SpbGUVM Publishing House</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.17238/issn2072-2419.2018.3.22</article-id><article-id custom-type="elpub" pub-id-type="custom">ivm-578</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИНФЕКЦИОННЫЕ БОЛЕЗНИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>INFECTIOUS DISEASES</subject></subj-group></article-categories><title-group><article-title>Оценка колонизации Ureaplasma diversum репродуктивной и респираторной систем коров</article-title><trans-title-group xml:lang="en"><trans-title>Assessment of U. Diversum carrier state in reproductive and respiratory system of cows</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ваганова</surname><given-names>А. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Vaganova</surname><given-names>A. N.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Фрейлихман</surname><given-names>О. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Freilikhman</surname><given-names>O. A.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Борисенко</surname><given-names>С. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Borisenko</surname><given-names>S. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Рока</surname><given-names>В. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Roca</surname><given-names>V. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Вербов</surname><given-names>В. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Verbov</surname><given-names>V. N.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФБУН НИИ эпидемиологии и микробиологии имени Пастера</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Saint-Petersburg Pasteur Institute</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>03</day><month>06</month><year>2021</year></pub-date><volume>0</volume><issue>3</issue><fpage>22</fpage><lpage>27</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Ваганова А.Н., Фрейлихман О.А., Борисенко С.В., Рока В.В., Вербов В.Н., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Ваганова А.Н., Фрейлихман О.А., Борисенко С.В., Рока В.В., Вербов В.Н.</copyright-holder><copyright-holder xml:lang="en">Vaganova A.N., Freilikhman O.A., Borisenko S.V., Roca V.V., Verbov V.N.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vetjournal.spbguvm.ru/jour/article/view/578">https://vetjournal.spbguvm.ru/jour/article/view/578</self-uri><abstract><p>Заболевания крупного рогатого скота, ассоциированные с Ureaplasma diversum ведут к экономическому ущербу, обусловленному бесплодием, абортами и рождением ослабленного потомства. U. diversum способна к колонизации слизистых оболочек репродуктивного и респираторного тракта взрослых животных и молодняка, при этом в большинстве случаев носительство протекает бессимптомно, что затрудняет контроль распространения инфекции. Целью данного исследования была оценка носительства U. diver-sum в носовой полости, влагалище и преддверии влагалища у взрослых коров. Для исследования были отобраны мазки с поверхности слизистых оболочек соответствующих отделов репродуктивной и респираторной систем у 32 животных. С целью выявления U. diversum в исследуемом материале применялась ПЦР в реальном времени. Наиболее распространённым вариантом носительства U. diversum была колонизация преддверия влагалища, выявленная у 48% обследованных животных. Однако ДНК U. diversum также выявлялась во влагалище и носовой полости обследованных коров. Было показано, что носительство уреаплазм в преддверии влагалища и носовой полости ассоциировано с колонизацией влагалища, а также, что колонизация респираторного тракта чаще выявляется у животных, являющихся носителями уреаплазм в половой системе. Полученные данные позволяют рекомендовать использование мазков из преддверия влагалища в качестве исходного материала при контроле данной инфекции. Носительство U. diversum в преддверии влагалища взрослых коров является фактором риска заражения молодняка при родах, а также колонизации других отделов репродуктивной системы, в том числе при искусственном осеменении.</p></abstract><trans-abstract xml:lang="en"><p>Cattle diseases associated with Ureaplasma diversum lead to economic burden caused by infertility, abortion and the birth of weakened offspring. U. diversum is capable to colonise the mucous membranes of the reproductive and respiratory systems cattle and calves, and most cases of carriage are asymptomatic, which makes it difficult to control the spread of the infection. The pur pose of this study was to evaluate frequency of U. diversum carriage in the nasal cavity, vagina and vestibule of vagina of adult female cattle. Smears from the surface of the mucous membranes of the reproductive and respiratory systems were sampled from 33 animals. Samples were tested for U. diver-sum DNA by real-time PCR. The most common variant of U. diversum carriage was the colonization of the vestibule of vagina, it was found in 48% of the examined animals. However, the DNA of U. diversum was also detected in the vagina and nasal cavity of the examined cattle. It was shown that the carriage of ureaplasma in the vestibule of vagina or nasal cavity was associated with colonization of the vagina, and that colonization of the respiratory tract is more often detected in animals that carry ureaplasma in the reproductive system. These data allow the use of smears from the vestibule of vagina as sample for the control and diagnosis of this infection. The carriage of U. diversum on the vestibule of the vagina in adult cows is a risk factor for calves infection during labor, as well as for colonization of other parts of the reproductive system, including contamination in artificial insemination. 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