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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">ivm</journal-id><journal-title-group><journal-title xml:lang="ru">Международный вестник ветеринарии</journal-title><trans-title-group xml:lang="en"><trans-title>International Journal of Veterinary Medicine</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2072-2419</issn><publisher><publisher-name>SpbGUVM Publishing House</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.52419/issn2072-2419.2022.4.60</article-id><article-id custom-type="elpub" pub-id-type="custom">ivm-966</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИНФЕКЦИОННЫЕ БОЛЕЗНИ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>INFECTIOUS DISEASES</subject></subj-group></article-categories><title-group><article-title>Молекулярная диагностика представителей рода Cryptosporidium в условиях промышленного свиноводства Вологодской области Северо-Западного федерального округа РФ</article-title><trans-title-group xml:lang="en"><trans-title>Molecular diagnostics of Cryptosporidium representatives in industrial pig industry in the Vologda region North-Western federal district of the Russian Federation</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7015-8063</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кряжев</surname><given-names>А. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Kryazhev</surname><given-names>A. L.</given-names></name></name-alternatives><bio xml:lang="ru"><p> профессор кафедры эпизоотологии и микробиологии, д.в.н., доцент </p></bio><bio xml:lang="en"><p> Professor, Department of Epizootology and Microbiology, Doctor of Veterinary Sciences, Associate Professor </p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-6919-8524</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Новиков</surname><given-names>А. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Novikov</surname><given-names>A. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p> доцент кафедры внутренних незаразных болезней, хирургии и акушерства, к.в.н., </p></bio><bio xml:lang="en"><p>Associate Professor, Department of Internal NonContagious Diseases, Surgery and Obstetrics, Ph.D.  </p></bio><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>ФГБОУ ВО Вологодская ГМХА им. Н.В. Верещагина</institution><country>Россия</country></aff><aff xml:lang="en"><institution>FSBE Institution of Higher Education «Vologda State Dairy Farming Academy named after N.V. Vereshchagin»</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2022</year></pub-date><pub-date pub-type="epub"><day>28</day><month>01</month><year>2023</year></pub-date><volume>0</volume><issue>4</issue><fpage>60</fpage><lpage>69</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кряжев А.Л., Новиков А.С., 2023</copyright-statement><copyright-year>2023</copyright-year><copyright-holder xml:lang="ru">Кряжев А.Л., Новиков А.С.</copyright-holder><copyright-holder xml:lang="en">Kryazhev A.L., Novikov A.S.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vetjournal.spbguvm.ru/jour/article/view/966">https://vetjournal.spbguvm.ru/jour/article/view/966</self-uri><abstract><p>Криптоспоридиоз – широко распространенная паразитарная болезнь многих видов домашних и диких животных, а также человека, являющееся значимой проблемой в области ветеринарии и медицины. Сельскохозяйственные животные, в частности поросята, наиболее часто подвержены данной болезни, однако видовой состав представителей рода Cryptosporidium у данного вида животных в РФ до настоящего дня оставался неизвестным. Впервые в Российской Федерации, в условиях северо-запада, на примере Вологодской области в свиноводческих хозяйствах с промышленной технологией содержания поросят при использовании новейших молекулярно-генетических методик, а именно с помощью высокопроизводительного секвенирования ампликонных библиотек фрагментов гена 18S рРНК, полученных в результате проведения nested (вложенной) ПЦР, нами установлено паразитирование С. scrofarum во всех возрастных группах обследуемых животных. Общая инвазированность поголовья в условиях свинокомплексов составила 34% (51/150). Поросята-сосуны были инвазированы криптоспоридиями в 40% случаев (12/30), интенсивность криптоспоридиозной инвазии была преимущественно сильной (+++), встречалась в 20% (6/30) случаев. Средняя (++) и слабая (+) степени инвазированности животных также имели место в 3% (10/30) случаев каждая. В возрастной группе поросят-отъемышей в возрасте 1-3 месяца инвазированность криптоспоридиями составила 33,3% (10/30), доминировала сильная (+++) степень выделения ооцист, она составила 26,7% (8/30) против 10 % (3/30) средней (++) и 3,3% (1/30) слабой (+). Наиболее инвазированы ооцистами криптоспоридий поросята-откормочники в возрасте 4-6 месяцев, экстенсинвазированность данной группы составила 60 % (18/30). Степень выделения ооцист была преимущественно средней (++) – 26,7% (8/30) и сильной – 23,3% (7/30). В 13,3% (4/30) случаев встречалась слабая (+) степень криптоспоридиозной инвазии. Животные старше 6 месяцев были заражены криптоспоридиями в 20% (6/30) случаев. У них регистрировали также среднюю (++) – 13,3% (4/30) и сильную (+++) – 6,7% (2/30) степени инвазии, слабой (+) инвазированности в данной возрастной группе не обнаруживали. Свиноматки также были инвазированы криптоспоридиями. Их зараженность составила 16,7% (5/30), а степень криптоспоридиозной инвазии была слабой (+).</p></abstract><trans-abstract xml:lang="en"><p>Cryptosporidiosis is a widespread parasitic disease of many species of domestic and wild animals, as well as humans, which is a significant problem in the field of medicine and veterinary medicine. Farm animals, in particular piglets, are most often susceptible to this disease, but the species composition of representatives of the genus Cryptosporidium in this species of animals in the Russian Federation has remained unknown to date. For the first time in the Russian Federation, in the conditions of the north-west, on the example of the Vologda region in pig farms with industrial technology for keeping piglets using the latest molecular genetic techniques, namely, using high-throughput sequencing of amplicon libraries of fragments of the 18S rRNA gene obtained as a result of nested PCR, we have established parasitism of C. scrofarum in all age groups of the animals examined. The total invasion of livestock in pig farms was 34% (51/150). Pine piglets were invaded by Cryptosporidium in 40% of cases (12/30), the intensity of Cryptosporidium infection was predominantly strong (+ + +), occurred in 20% (6/30) of cases. The mean (+ +) and weak (+) degree of animal invasion also occurred in 3% (10/30) of cases each. In the age group of weaning piglets at the age of 1-3 months, Cryptosporidium infection was 33.3% (10/30), a strong (+ + +) degree of oocyst production dominated, it was 26.7% (8/30) versus 10% (3/30) of the average (+ +) and 3.3% (1/30) weak (+). The most invasive oocysts are Cryptosporidium of fattening piglets at the age of 4-6 months, the extensinvasion of this group was 60% (18/30). The degree of oocyst release was predominantly moderate (+ +) – 26.7% (8/30) and strong – 23.3% (7/30). In 13.3% (4/30) of cases, there was a weak (+) degree of Cryptosporidium infection . Animals older than 6 months were infected with Cryptosporidium in 20% (6/30) of cases. They also recorded an average (+ +) – 13.3% (4/30) and a strong (+ + +) – 6.7% (2/30) degree of invasion, weak (+) invasion in this age group was not detected. Sows have also been invaded by Cryptosporidium. Their infection rate was 16.7% (5/30), and the degree of cryptosporidiosis was weak (+).</p></trans-abstract><kwd-group xml:lang="ru"><kwd>криптоспоридиоз</kwd><kwd>Cryptosporidium scrofarum</kwd><kwd>ооцисты</kwd><kwd>ПЦР</kwd><kwd>ДНК</kwd><kwd>секвенирование</kwd><kwd>18S рРНК</kwd><kwd>поросята</kwd><kwd>Вологодская область</kwd><kwd>Российская Федерация</kwd></kwd-group><kwd-group xml:lang="en"><kwd>cryptosporidiosis</kwd><kwd>Cryptosporidium scrofarum</kwd><kwd>oocysts</kwd><kwd>PCR</kwd><kwd>DNA</kwd><kwd>sequencing</kwd><kwd>18S rRNA</kwd><kwd>piglets</kwd><kwd>the Vologda region</kwd><kwd>the Russian Federation</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Исследование выполнено за счет гранта Российского научного фонда № 22-26-00002, https://rscf.ru/project/22-26-00002/</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Adl, S.M. 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