Consolidation of experimental fractures during intramedullary osteosynthesis in rats using Collapan-L and Thymogen

Abstract

The article presents the results of treatment of experimental femur fractures of laboratory animals (outbred rats) against the background of the use of a biocomposite material based on synthetic hydroxyapatite, collagen and lincomycin hydrochloride (collapan-L) and an immunotropic drug from the group of synthetic dipeptides (thymogen).
During the experiment, biochemical, radiological and histological studies were carried out. It was shown that during the first week after the fracture, there were no significant changes in the groups. While for the period from 7 to 21 days (according to X-ray data), processes of reparative osteogenesis proceeded more intensively (on the 14^th day) in the 1st, 4th, and 5th groups than it did in the control, and 2nd and 3rd experimental groups. These changes are consistent with biochemical blood tests. The radiographs obtained on the 28th day had no significant differences.
On the 45th day after the surgery, the fibrocartilage callus forming in the group where both drugs were used simultaneously (thymogen ‒ immediately after the surgery) proceeded more pronouncedly compared to other groups. Thus, on radiographs, the fracture line was barely noticeable, which indicates more actively occurring processes of osteogenesis. These assumptions are also consistent with the level of calcium in the blood serum, as well as with histological studies. Thus, on the 45th day after the surgery, the fibrocartilage callus consisted of 60.2 ± 2.1% of bone tissue, which was 4.9% higher than in the control group and 9.39% higher than the impact of the mechanical factor (rupture resistance).
Thus, the results of the studies on rats with experimental femoral fractures made it possible to establish that the combined use of the tested drugs contributes to a more intensive process of reparative osteogenesis (in the 4th experimental group).