The study of R- brucella colored antigen in farms with different epizootic status

In the pathogenesis of brucellosis, L-formulas of brucella have a certain value, which persist for a long time in the body. In the chronic process, the cultures of brucella of stable L-forms were isolated from animals. The aim of the study was to determine the diagnostic significance of the experimental series of R-brucellosis color antigen in the agglutination reaction. The diagnostic value of R-brucellosis diagnosticums was studied using the example of farms in the Omsk region with different epizootic status of territories: with unvaccinated livestock (n = 270), vaccinated livestock (n = 145), healthy livestock (n = 909) of cattle. Diagnostics were used from commercial kits, constructed from S-forms of brucella for RA, RSK, RID, RNGA, BPD. Reactions were made in accordance with the Manual on the use of antigens. The sensitivity test of R-brucellosis antigens in RA and DAC was performed in a brucellosis-free household (145 samples) 3 to 4 months after immunization with animals from the strain Br.abortus 82. The production test of the diagnosticum showed the advantages of the antigen proposed by us: 1) specificity and pronounced sensitivity in the early stages of studies on brucellosis, which is especially valuable in the study of immunity control in vaccinated livestock. With its help, it is possible to further reveal from 5.6 to 15.8% of animals with postvaccinal R antibodies that were not serologically diagnosed in the reaction of RCC with R antigen; 2) the possibility of using it for epizootic assessment of the herd as a differential test of postvaccinal reactions in immunized animals with weakly agglutinogenic vaccines in (SR, RS forms).

крупный рогат ый скот , бруцеллез, ант ит ела, ант иген, R- форма, cattle, brucellosis, antibodies, antigen, R form

1. Аракелян, П.К. Роль R-антигенов в дифференциальной поствакцинальной диагностике бруцеллеза крупного рогатого скота, иммунизированного живыми слабоагглютиногенными вакцинами / Аракелян П.К., Разницына Г.В., Янченко Т.А. //Достижения науки и техники АПК. - 2015. - №2. - С. 63-66.

2. Дегтяренко, Л. В. Методы иммунологической оценки животных, сенсибилизированных измененными формами бруцелл / Дегтяренко Л. В., Гордиенко Л. Н., Власенко В. С. // Методическое пособие. - 2017. - С. 30

3. Наставление по диагностики бруцеллеза //МСХ РФ.-М.-2003.- С. 60. 4.Электронный ресурс Минсельхоза Казахстана- URL: https: // ru.sputniknews.kz / regions / 20180213 /4583529/ gde-v-kazahstane-est-ochagi-brucelleza-rasskazali-v-minselhoze.html (дата обращения 28.06.2018)

4. Эпизоотическая ситуация в РФ // Информационный аналитический центр Россельхознадзора [Электронный ресурс]. - URL: http://www.fsvps.ru/fsvps/ iac/rf/operative-messages (дата обращения 09.04.2018)