THIN-LAYER CHROMATOGRAPHY AS A METHOD OF DETECTING PESTICIDES IN POULTRY PRODUCTS

The article describes the application of thin-layer chromatography method for the detection of synthetic pyrethroids in hen's eggs obtained in poultry farm egg production facilities located in the Leningrad region after insecticidal treatment with 1% s-biotrin solution. Chromatography was carried out according to the adapted methodology with the use of UVK-HDi ultraviolet cabinet, the company “Petrolaser” Ltd. Sample preparation was based on the analysis of literature sources and the experience of the experiments conducted earlier. For this purpose, the egg contents were homogenized and subjected to extraction with the addition of organic solvent acetone. The obtained extract was centrifuged at 1000 rpm. The precipitate was dehydrated in a desiccator until the lowest mass was reached. No pesticide content was detected in chicken egg samples collected from the insecticide treated poultry house. The results were consistent with those of samples from a control poultry house that had not been treated. The chromatography results of chicken egg samples from production facilities were compared with the results of eggs experimentally treated with a 1% insecticide solution. Solutions of the active substance at the same concentration were used as a control to evaluate the chromatography results. Substance stains on the plates corresponded to the values of pure substance solution samples. The mobility coefficient of s-biotrin was 0.31±0.03. The method of thinlayer chromatography is an accessible and indicative method of qualitative analysis of the content of residual concentrations of synthetic pyrethroids and disinsection efficiency. The use of modern equipment and materials of domestic production makes this method available for laboratories and research organizations of various levels, which demonstrates the relevance of the development of this technique in the context of the need for import substitution.

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