Evaluation of the effect of the complex adsorbent "Galluasorb" on the ultrastructure of poultry liver in mycotoxicosis

Despite the development and modernization of agriculture, mycotoxicosis remains one of the important problems of poultry farming. Due to the production of several mycotoxins by mold fungi at once, it is important to talk about their combined action, which, due to the synergistic effect, can cause more serious changes in the body than each individual toxin. Pathological changes primarily occur in the liver and other target organs. It is known that drugs that have a targeted effect on hepatocytes are promising as therapeutic and prophylactic agents against mycotoxicosis. The objective of this work is to evaluate the hepatoprotective activity of the complex adsorbent "Galluasorb" at the ultrastructural level in experimental combined mycotoxicosis of broiler chickens. The experiment was conducted on 40 broiler chickens of the СOBB-500 cross, aged 21-22 days, divided into four groups: group 1 (BC) - biological control; group 2 (TC) – toxic control (toxic diet contaminated with T -2 toxin, aflatoxin B1 and zearalenone); group 3 (TC+GS) – preventive (toxic diet with the addition of 0.25% Galluasorb); group 4 (BC+GS) – safety control (regular diet with 0.25% Galluasorb). For morphological examination, 1 mm3 liver pieces were prepared using the ultrathin section technique, contrasted and examined on TEM. Then, the obtained electronograms were described and morphometry was performed, taking into account the following mitochondria characteristics: area, perimeter, caliperometric diameter (Feret diameter), roundness coefficient. Statistical data processing was performed using descriptive and comparative statistics (nonparametric Mann-Whitney test, α=0.05; Bonferroni correction p). Based on the research results, a protective effect of the Galluasorb adsorbent was revealed in relation to the general cytomorphology of hepatocytes and morphometric indices of mitochondria of hepatocytes in broiler chickens.

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