Development of latex agglutination reaction for the diagnosis of hemorrhagic enteritis in turkeys

With the rapid growth rate of the turkey breeding industry and the emergence of new viral diseases, we have to face the problem of the lack of diagnostic kits and methods of specific prevention of these diseases in Russia. The most common and economically dangerous immunosuppressive disease is hemorrhagic enteritis of turkeys. There are no kits available for the diagnosis of the GEI virus in Russia, which is why the purpose of our work was to develop a serological diagnostic method using the latex agglutination reaction as an example. 4 groups were created with the difference that different buffer solutions were used for diluting and washing latex, different types of latex, their size, as well as the type of purification of the globulin fraction (the first method using PEG 6000, and the second by precipitation of the globulin fraction with ammonium sulfate followed by dialysis). After deposition of the globulin fraction on latex microspheres, according to the method described in the article, checks were carried out in the following way: latex diagnosticum in a volume of 50 ml was dripped onto plastic and a supernatant of homogenate of turkeys infected with the GEI virus was added to it, and 50 ml of latex diagnosticum was dripped with the addition of a supernatant of homogenate of spleens of uninfected turkeys. The ingredients were mixed using a wooden stick and waited 10 minutes to account for the reaction. After taking into account the reaction, it was concluded that the best modification of latex is carboxylated with a diameter of 200-400 nm, the best buffer is tris-salt, and the best dilution of the globulin fraction is 1:2-1:16.

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