Determination of the concentration of inflammatory and anti-inflammatory interleukines in dairy cows with infectious mastitis of staphylococcal etiology

The etiology of mastitis is associated with a high percentage (90 %) of isolation of bacteria of the genus Staphylococcus. According to statistics, the problem of mastitis in lactating cows is relevant, since it is associated with the economic costs of treatment and diagnosis. Modern diagnosis of infectious mastitis, based on biomarkers (interleukins) of the inflammatory process, will allow assessing the course and form of mastitis, and significantly reduce the economic costs of diagnosis. The purpose of the study was to determine the concentration of pro-inflammatory (IL-1 β, IL-2, IL-6, IL-8) and anti-inflammatory (IL-4, IL-10) interleukins, in milk and blood serum, in different groups of dairy cows, so that the obtained concentration values could be used as predictive biomarkers of the course of infectious mastitis of staphylococcal etiology. To conduct the study, 3 groups of 10 heads of lactating red-and-white Holstein dairy cows were formed. 10 milk samples and 10 blood samples were taken from each group of dairy cows: clinically healthy, with subclinical and clinical mastitis. The basis for the division into groups was the rapid test for mastitis. To determine the concentration of pro-inflammatory and anti-inflammatory interleukins in serum and milk, the enzyme-linked immunosorbent assay method and the corresponding set for determining the concentration of a particular interleukin were used. The obtained values of concentrations in milk were promising, in particular, IL-2 was increased by 9,9 times in the group of clinically ill mastitis compared to the group of clinically healthy and IL-6 in the group of clinically ill mastitis by 9,5 times compared to the group of clinically healthy cows. Serum interleukin concentrations were increased: for IL-2, 4.2 times between the group of clinically ill mastitis and the group of clinically healthy cows, and IL-6 concentration in the group of clinically ill mastitis was 9,5 times higher compared to the group of clinically healthy cows.

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