Rationale for the use of testicular hyaluronidase in the therapy of bovine mastitis

The study evaluated the effectiveness of testicular hyaluronidase in the treatment of mastitis in cows. The research was conducted on Holstein animals with recurrent catarrhal inflammation of one udder quarter (more than twice per lactation). All animals (n=20) underwent therapy, which included a supramammary novocaine block and an injection of an anti-inflammatory agent on the first day of treatment, as well as intracisternal administration of the drug «Mastiet Forte» until clinical recovery. The experimental group (n=10) additionally received an intracisternal injection of 1280 units of hyaluronidase diluted in 5 ml of saline solution. The results showed that in the experimental group, compared to the control, the somatic cell count was lower by 115.2 thousand/ml, and the number of animals with an inflammatory reaction cell concentration in the secretion exceeding 500 thousand/ml was lower by 20 %. The electrical conductivity of milk in the cows of the control group decreased by 28,6 %, while in those receiving the enzyme it decreased by 34,5 %, which is 22,1 % less compared to the animals receiving standard treatment (P≤0,01). The course of intracisternal administrations when using hyaluronidase was shortened by 0,9 days. During the one-month observation period, a relapse of the pathology was noted in one cow from the experimental group compared to three in the control group. Hematological analysis revealed that therapy with the enzyme promoted active resolution of persistent inflammation: a decrease in the number of monocytes and eosinophils, and a shift in the leukogram towards neutrophilic leukocytes, whereas in the control group these changes were less pronounced. Thus, the use of testicular hyaluronidase in the complex treatment of recurrent mastitis in cows increases the clinical effectiveness of the therapy.

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