Assessment of U. Diversum carrier state in reproductive and respiratory system of cows

Cattle diseases associated with Ureaplasma diversum lead to economic burden caused by infertility, abortion and the birth of weakened offspring. U. diversum is capable to colonise the mucous membranes of the reproductive and respiratory systems cattle and calves, and most cases of carriage are asymptomatic, which makes it difficult to control the spread of the infection. The pur pose of this study was to evaluate frequency of U. diversum carriage in the nasal cavity, vagina and vestibule of vagina of adult female cattle. Smears from the surface of the mucous membranes of the reproductive and respiratory systems were sampled from 33 animals. Samples were tested for U. diver-sum DNA by real-time PCR. The most common variant of U. diversum carriage was the colonization of the vestibule of vagina, it was found in 48% of the examined animals. However, the DNA of U. diversum was also detected in the vagina and nasal cavity of the examined cattle. It was shown that the carriage of ureaplasma in the vestibule of vagina or nasal cavity was associated with colonization of the vagina, and that colonization of the respiratory tract is more often detected in animals that carry ureaplasma in the reproductive system. These data allow the use of smears from the vestibule of vagina as sample for the control and diagnosis of this infection. The carriage of U. diversum on the vestibule of the vagina in adult cows is a risk factor for calves infection during labor, as well as for colonization of other parts of the reproductive system, including contamination in artificial insemination. ЛИТЕРАТУРА

Ureaplasma diversum, уреаплазмоз КРС, заболевания репродуктивной системы КРС, ПЦР, Ureaplasma diversum, ureaplasmosis of cattle, reproductive diseases of cattle, PCR

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