Use of the dye of brilliant cresil blue for diagnostic of babesiosis of dogs

Diagnosis of babesiosis according to the results of studies of fixed and stained blood smears and currently remains one of the main methods nowadays. Therefore, the search for alternative dyes to reduce the time of staining blood smears, increase their diagnostic quality, reduce the cost of diagnostic measures, adapt the research methodology for production conditions, and also reduce the toxic effect of reagents on the researcher is relevant. The paper presents materials on the use for the diagnosis of babesiosis of animals of an alternative vital dye, which is brilliantcresil blue. Babesia staining using this dye is possible according to one of two proposed methods. The first technique involves staining by adding 1 drop of concentrated dye to a test tube containing 0.5-1.0 ml of stabilized anticoagulant blood and preparing a smear 7-10 minutes after that. The second technique can be performed immediately on a glass slide by mixing 1 drop of dye and 1 drop of blood not containing an anticoagulant and preparation of a smear, which must be placed in a moist chamber for 7-10 minutes. Babesia, when stained with brilliantcresil blue, acquire a dark blue color inside light yellow, light green or light pink red blood cells. Among these methods, the proposed method of coloring is easy to perform, since it does not require the use of toxic fixatives, preparation of solutions, which reduces the time spent on research. At the same time, this staining method has high diagnostic efficiency, providing a clear visualization of babesia in both red blood cells and blood plasma, eliminates the appearance of artifacts, and, accordingly, false positive or false negative results. The use of this method and dye is economically advantageous not only for single studies, but also especially for mass examinations and medical examination of animals. The proposed method of stain suggests a real opportunity to perform mass diagnostic tests in a production environment.

бриллиант овыйкрезиловый синий, диагност ика бабезиоза, окраска ретикулоцитов, brilliantcresylblue, diagnosis of babesiosis, reticulocyte staining

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