Study of acute toxicity of hepatoprotector "hepaton " in rodents

The aim of this work was to study in laboratory conditions the harmlessness of the hepatoprotector "Hepaton", which is an emulsion of light brown shade for oral use, including milk Thistle oil, tocopherol acetate, ursode-oxypholic acid, methionine, rose hip syrup. Due to the active substances, the drug exhibits immunomodulatory, hypocholesterolemic, hepatoprotective, choleretic, cholelitolytic effect. For the experiment, 4 groups of control and experimental mice and rats with 10 heads each (the groups included both males and females) were formed. Experimental animals were injected with hepatoprotector, after which toxicometry was carried out [GOST 32296-2013] [1, 3], through the study of lethal dose, analysis of rodent body weight, as well as necropsy. During the experiment, there were no obvious differences in the General condition and behavior of rodents. Barely noticeable delayed reaction and loss of appetite in the first two days were observed in all groups of animals, and are associated, most likely, not with the toxic effect of the studied drug, but with a stress factor as a result of the procedure of repeated administration of Hepatone in large volumes. Hepatoprotector "Hepaton" when administered intragastrically in doses up to 50 ml / kg to mice and rats did not lead to death and did not cause any macroscopic changes in internal organs. At necropsy there was no hypervolemic edema of organs, as indicated by the values of their mass coefficients. Also, there was no irritant effect on the mucous membranes of the gastrointestinal tract. The conducted experiment testifies to harmless influence of Hepatone on an organism of mice and rats, and also about low toxicity of the applied preparation that is extremely important for further researches on influence of hepatoprotector.

острая токсичность, интоксикация, масса тела, некропсия, грызуны, acute toxicity, intoxication, body weight, necropsy, rodents

1. Андреева Н.Л., Лунегов А.М., Барышев В.А., Глушкова О.С., Большаков К.И. Учебное пособие к практическим занятиям по фармакогнозии для студентов ветеринарного факультета очной и заочной формы обучения. - СПб, 2017 г.-147 с.

2. Васькова, Л. Б. Методы и методики фармакоэкономических исследований : учеб. пособие / Л. Б. Васькова, Н. З. Мусина. - Москва : ГЭОТАР-Медиа, 2007. - 112 с.

3. ГОСТ 32296-2013. Методы испытаний по воздействию химической продукции на организм человека. Основные требования к проведению испытаний по оценке острой токсичности при внутрижелудочном поступлении методом фиксированной дозы. Межгосударственный стандарт.

4. ГОСТ 33216-2014. Руководство по содержанию и уходу за лабораторными животными. Правила содержания и ухода за лабораторными грызунами и кроликами. Межгосударственный стандарт.

5. Оценка клинического состояния животных и применение лекарственных препаратов при болезнях пищеварительного аппарата/ А.В. Яшин, Г.В. Куляков, Г.Г. Щербаков, А.М. Лунегов, В.А. Барышев, И.И. Калюжный. -СПб.; Саратов, 2019.-160 с.

6. ГОСТ ISO 10993-11-2011 Изделия медицинские. Оценка биологического действия медицинских изделий. Часть

7. Исследования общетоксического действия