Influence of phytobiotics on the microbiota of the intestinal calves

Abstract

A study of the antimicrobial activity of the new drug was carried out in vitro by diffu sion into agar for the following reference strains of microorganisms - Enterococcus feacalis, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus. For the study, a suspension was prepared containing a standard number of microorganisms, which was sown on a layer of nutrient agar in Petri dishes. The size of the seed surface was determined according to the procedure described in GFXIII.To study the effect of calves on the intestinal microbiota, a new phytosorption complex, 2 groups of calves were formed, whose age was 2-3 months, of a black-and-white breed. A scientific and production experiment was carried out in the SEC “Kolkhoz Leninsky Put” of the Pushkinogorsky district of the Pskov region, which has a milk direction. The average weight of the experimental group at the time the experiment began was 79.2 ± 8.53 kg, and the control 81.6 ± 9.42 kg. An experimental group of calves was administered a phytosorption complex at a dose of 120 gr/ animal, which corresponds to 1.5 g / kg. The drug was prepared daily, dissolved in 1.5-2 liters of warm water and given orally to the animals for 14 days. The control group received a standard diet, without any additives. At the beginning of the experiment, and at the end, samples of fecal matter were taken from the large intestine for bacteriological studies by plating on differential media.The results of a production experiment proved the positive effect of the phytosorption complex on calf intestinal microbiocinosis. So it is possible to note an increase in lactobacilli and bifidobacteria in the experimental group by 35 and 21.6%, a slight increase was noted, by 2.17% in Escherichia coli. Analyzing the data obtained, we can conclude that the addition of biologically active substances to the diet of calves contributed to an increase in production indicators. Thus, the addition of a phytosorption complex to calf feed contributed to an increase in weight in relation to the control group by 7.8%.