The exocrine pancreatic function in laying hens fed different calcium levels

The excretory activity of the digestive system is closely correlated with other aspects of the activity of the digestive glands including: secretion and metabolism of intermediate substances. The studies of this activity are therefore urgent and require new approaches. The aim of the study was to investigate exocrine pancreatic function in laying hens, fed diets with different calcium (Ca) level. The experiment was performed on three Hisex White hens with chronic fistulae of main pancreatic duct. The method of periods was used; dietary Ca content during control period was 3.6%, during experimental period I - 3.4%, experimental period II - 4.2%. The changes in dietary Ca content were found to affect the exocrine pancreatic function; the evidence to these changes were stated via the increases in the secretion volume and changes in the activities of lipase and total proteases. The effects of higher Ca dose were stated during the complex-reflex phase of regulation of the pancreatic secretion: during the diet with higher Ca level the activity of total proteases increased during the first postprandial minutes in comparison to the control period. On the contrary, during the diet with lower Ca level, this activity decreased during the neurohumoral phase of the pancreatic regulation, in 90 min after the feeding. The dynamics of the activity of alkaline phosphatase (AP) in pancreatic juice during the postprandial period was reversed in compare to proteases: this activity abruptly decreased immediately after the feeding. The changes in dietary Ca level affected the basal levels of AP activity; postprandial activity levels were similar to those for the control period. The negative correlations were found between the activity of total proteases and activity of AP (r=-0.87) and Ca content in pancreatic juice (r=-0.64), while the activity of AP and Ca content in pancreatic juice correlated positively (r=0.81).

экзокринная функция подж елудочной ж елезы, куры, панкреатические ферменты, щелочная фосфатаза, кальций, exocrine pancreatic function, laying hens, pancreatic enzymes, alkaline phosphatase, calcium

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