Dynamics of white blood under femoral bone fractures in rats on the background of application of RV-2 immunomodulator and RVI bio-compositional material

Abstract

The paper presents data on the dynamics of some indicators of white blood in outbred white rats during osteosynthesis of tubular bones on the background of the application of the RVI biocomposite material preparation and the RV-2 immunomodulator from the group of synthetic dipeptides. The experiments were performed on 30 animals, divided into six groups (n=6 in each). The rats of the control group after osteosynthesis were not treated (untreated wound model), experimental rats from the 1st, 2nd, 3rd, 4th and 5 th groups were treated with the preparations separately or in combination at various times after surgery (immediately or 5 days after). Blood for the study was sampled before surgery, and then with a weekly interval. The duration of the experiment was 21 days. It was shown that on the early stages of oste ogenesis at the first and second phase, bone healing during the first 14 days had been accompanied by an increase in the number of leukocytes, lymphocytes and granulocytes in animals of the control group, as well as of the 1st, 2nd, 3rd and 5th experimental groups treated with RVI and RV-2, but this indicator decreased in the 4th group (the combination of RVI and RV-2 was used immediately after surgery). In the subsequent periods of action of the preparations were of multidirectional nature. Comparing the final data of the experiments to the background it should be noted, that in the control group of animals the absolute numbers of leukocytes, lymphocytes and granulocytes reached only 91.2; 84.3 and 89.5%, with 2.5 times increase in the content of cells of medium size (MID); in the 1st and 4th experimental groups, the data were similar in values, differing only in relation to the number of MID cells - 87.0; 91.8; 80.6; 50.0 % and 71.6; 64.0; 96.7; 75.0 %, respectively. More pronounced results were obtained with the use of RV-2 (separately or 5 days after the surgery, in combination with the RVI preparation), where the ratio of the number of leukocytes, lymphocytes and granulocytes to background indicators in the 2nd group was 113.4; 113.2; 79.4 % by 7 times increasing the number of MID cells; in the 3rd -105.3;103.2; 75.0 % by 3,8 times; in the 5th - 121.1; 102.9; 200.0% with 2.5 times decrease in the number of cells of medium size. The obtained data should be considered as advisory when choosing preparations for practical use.