Morphofunctional changes in the reproductive organs of females against the background of the use of a phytopreparation (experimental study)
https://doi.org/10.52419/issn2072-2419.2023.4.133
Abstract
The paper presents the results of the assessment of morphological changes in the uterus and ovaries of laboratory animals against the background of the introduction of therapeutic doses of a multifunctional phytopreparation, which includes alcohol extracts from plants: white hellebore (Veratrum album), dioecious nettle (Urtica dioica), yarrow (Millefolii herba) and has rumination, uterotonic, hemostatic and anti-inflammatory effects. Therapeutic doses for cows were tested, which for rats amounted to 0.2 ml per head. The phytopreparation was adminitered subcutaneously three times, every other day. The control group rats were subcutaneously injected with a saline solution of sodium chloride (NaCI 0.9%) according to the same scheme. The methods of digital microscopy were used in the study of micropreparations. Morphometric analysis of the histoarchitectonics of the uterus revealed a statistically significant increase in the diameter of the cross-section of the uterus of female rats of the experimental group by 25.8% (p≤0.05) compared with the control group due to an increase in the epithelial by 24.5% (p≤0.05) and the endometrial lamina of the uterine mucosa by 32% (p≤0.05), which indicates on increasing the secretory activity of the epithelial cover of the uterus. In the experimental group of rats treated with phytopreparation, a significant increase of 46% (p<0.05) in the number of primordial follicles was noted. There was also a tendency to increase the number of primary – by 36.3%, secondary – by 41.7%, tertiary – by 75% of follicles and yellow bodies – by 22.2% in rats of the experimental group compared with the control indicators. Quantitative indicators of the follicular composition of the ovaries of rats of the experimental group and an increase in the follicle aging coefficient by 60% (p≤0.05) indicate stimulation of the ovarian reserve against the background of the use of phytopreparation.
About the Authors
T. V. Boyko
Omsk State Agrarian University named after. P.A. Stolypin
Russian Federation
Russian Federation
Doctor of Veterinary Sciences, Associate Professor, Head of the Department of Diagnostics, Internal Noninfectious Diseases, Pharmacology, Surger and Obstetrics
I. V. Ogurnoy
Omsk State Agrarian University named after. P.A. Stolypin
Russian Federation
Russian Federation
asp.
M. V. Koshkarev
Omsk State Agrarian University named after. P.A. Stolypin
Russian Federation
Russian Federation
Candidate of Medical Sciences. veterinarian. sciences, assoc.
References
1. Balagula T.V., Lavrukhina O.I., Batov I.V., Makarov D.A. Tretyakov A.V. Antibiotics in veterinary medicine: contamination of livestock products / – St. Petersburg 2022, No. 4. International Bulletin of Veterinary Medicine. From 174-180. DOI.org/10.52419/issn2072-2419.2022.4.174
2. Boyko T.V., Veretennikova V.S., Luksha E.A. A remedy for the treatment and prevention of postpartum diseases in cows / Russian Federation patent for invention No. 2719225. -2020 – Electronic resource. - Access point. -https://www.elibrary.ru/item.asp?Id=42712292
3. Boyko T.V., Ogurnoy I.V., Dochilova E.S. Morphofunctional changes in the organs of the reproductive system of females in pathology due to the use of herbal medicines (review) // Bulletin of OmSAU. 2022. No. 4 (48). URL: https://cyberleninka.ru/article/n/morfofunktsionalnye-izmeneniya-v-organah-reproduktivnoy-sistemy-samok-pri-patologii-na-fone-primeneniya-fitopreparatov-obzor (date of access: 07/24/2023). DOI 10.48136/2222-0364_2022_4_94
4. Galchenko V.A., Lisichenko G.O. Determination of the effectiveness of ultrasound examination of the ovaries in synchronizing sexual heat in cows / V.A. Galchenko, O.G. Lisichenko – St. Petersburg 2022, No. 1. International Bulletin of Veterinary Medicine. S180-185. DOI.org/10.52419/issn2072-2419.2022.1.180
5. Gorpinchenko E. A., Koba I. S., Lifentso-va M. N. Factors contributing to the occurrence of functional disorders of the reproductive apparatus in cows // Scientific journal of KubSAU. 2016. No. 121. URL: https://cyberleninka.ru/article/n/faktory-sposobstvuyuschie-vozniknoveniyu-funktsionalnyh-rasstroystv-rodopolovogo-apparata-u-korov (date of access: 07/24/2023). DOI: 10.21515/1990-4665-121-112
6. Kamernitsky A.V., Levina I.S., Kulikova L.E., Milovanov A.P. Morphological changes in rat uterine tissue under the influence of progestins and antiprogestins of the pregna-ya-pentaran series / Bioorganic chemistry, 2002, volume 28, no. 3, p261-268. DOI: 577.127.2:611.66:618.63
7. Kireev I.V., Pyanov B.V. The use of an antioxidant anti-inflammatory drug in complex treatment regimens for endometritis in cows / I.V. Kireev, B.V. Pyanov - St. Petersburg 2019, No. 3. International Bulletin of Veterinary Medicine. S22-25. DOI: 636.2.034:616-008.9
8. Kobaidze E.G., Padrul M.M. Endometrial dysfunction in chronic inflammation of the uterus // Perm Medical Journal. 2014. No. 5. URL: https://cyberleninka.ru/article/n/narushenie-funktsii-endometriya-pri-hronicheskih-vospaleniyah-matki (date of access: 03/20/2023). DOI: 145.008.6.02:618.14-002.2
9. Kotelnikov A.V., Kotelnikova S.V. Characteristics of the estrous cycle of white rats at different stages of ontogenesis with the introduction of vitamin E // Vestnik ASTU. 2005. No. 3. URL: https://cyberleninka.ru/article/n/harakteristika-estralnogo-tsikla-belyh-krys-na-raznyh-etapah-ontogeneza-pri-vvedenii-vitamina-e (access date: 03/20/2023). DOI: 577.161.3:591.8:612.65
10. Lobanov, V. S. Histological structure of the pig uterus under the influence of progesterone / V. S. Lobanov, A. V. Filatov // Youth science - development of the agro-industrial complex: Materials of the All-Russian (national) scientific-practical conference of students, graduate students and young people scientists, Kursk, December 03–04, 2020. Volume Part 2. – Kursk: Kursk State Agricultural Academy named after I.I. Ivanova, 2020. – P. 195-200. – EDN NBQSWO.
11. Lunegov, A. M. Quaestio de pharmaceutica pollutione circa ambitum / A. M. Lunegov // Theoria et praxis pharmaceuticae veterinariae, ecologiae et toxicologiae in complexu agro-industriali: materias scientificae et practicae collationis internationalis in C anniversario Dicasterii dicatae. St. Petersburg, St. Petersburg, St. Petersburg, p. - St. Petersburg: St. Petersburg. -EDN SULESM
12. Prospects for solving obstetric and gynecological pathology in cows on an industrial farm / A. N. Turchenko, I. S. Koba, E. N. Novikova [etc.] // Proceedings of the Kuban State Agrarian University. – 2012. – No. 34. – P. 194-196. – EDN OQOYQP.
13. Lyzikova Yu. A. Chronic endometritis as a cause of individual reproductive function in women // Problems of health and ecology. 2015. No. 3 (45). URL: https://cyberleninka.ru/article/n/hronicheskiy-endometrit-kak-prichina-narusheniy-reproduktivnoy-funktsii-u-zhenschin (date of access: 03.20.2023). DOI: 618.14-002-036.12
14. Maltseva, L. I. Chronic endometritis and pelvic pain / L. I. Maltseva, G. R. Smolina, E. Yu. Yupatov // Obstetrics. Gynecology. Reproduction. - 2012. - T. 6, No. 3. - P. 23– 27. DOI: 10.17116/2015154102-105
15. Chuev S.A., Ryadinskaya A.A., Lavrienko K.V. et al. Physiological and biochemical indicators when stimulating the reproductive function of cows. Monograph / Electronic resource. - Access point. - https://books.google.ru/books?hl
16. Lyu, J. Antibiotics in soil and water in China – a systematic review and source analysis / J. Lyu, L. Yang, L. Zhang, B. Ye, L. Wang // Environ. Pollut. – 2020. – V. 266. Part 1. – 115147. DOI:org/10.1016/j.envpol.2020.115147
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For citations:
Boyko T.V., Ogurnoy I.V., Koshkarev M.V. Morphofunctional changes in the reproductive organs of females against the background of the use of a phytopreparation (experimental study). International Journal of Veterinary Medicine. 2023;(4):133-142. (In Russ.) https://doi.org/10.52419/issn2072-2419.2023.4.133
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