Dependence of coagulation activity of chicken blood IN VITRO on temperature

Abstract

The paper presents the results of examine  the  relationship  between  blood  plasma incubation  temperature  and  the  hemostasis parameters in chickens. The purpose of this research was to study of the blood coagulation  parameters  in  chickens  during  hypo-, normo- and hyperthermia in vitro. In addition, to assess the possibility of using a coagulometer to identify hemostasis activity and the use of hemostatic reactions in chickens as models for human medicine. The Vologda region was where the study was conducted. The coagulogram was examined for the following parameters: thrombin time (TT), prothrombin time (PT), activated partial thromboplastin  time  (APTT),  fibrinogen  activity, and antithrombin III activity (AT-III). It was found that at 46° C activation of blood clotting occurs, manifested as an acceleration of the  TT  by  29%  (13  seconds),  PT  by  72% (111  seconds),  and  decrease  in  the  antithrombin III activity by 6% (1.5 seconds) compared to normothermia. The parameters of  secondary  hemostasis  also  responded  to hypothermia (18 °C) with hypercoagulation. It  was  an  acceleration  of  PT  of  81%  (125 sec),  an  increase  in  fibrinogen  activity  by 77% (84 sec) and a decrease in AT-III activity  by  55%  (14  sec),  with  the  exception  of thrombin time, which was extended by 70% (104  seconds).  There  were  no  significant changes  in  the  APTT  at  different  temperatures.  The  correlation-regression  and  one-factor analysis of variance revealed a significant correlation between the temperature and the TT and the fibrinogen activity, where the coefficients of determination for which were 71%  and  39%,  respectively.  Plasma  hemostasis  activity  measured  on  a  coagulometer (at 37 °C) showed no significant differences from  those  measured  at  43  °C,  except  for prothrombin time, which was 71.51 seconds (47%) less than that measured at 43 °C.