The genetic basis of the sperm quality of stallions
https://doi.org/10.52419/issn2072-2419.2023.4.413
Abstract
One of the important factors affecting the reproductive function is the genotype of the animal. The method of genomic selection is based on the use of polymorphic single nucleotide substitutions (SNP) as markers for determining the value of the genotype of an animal or plant. The formation and functioning of the reproductive system of animals occurs as a result of the coordinated interaction of a wide range of genes. The search for genomic associations of GWAS (Genome-Wide Association Studies) with signs of fertility will allow further selection of animals based on these indicators at the genomic level. Sperm was obtained with the help of mares in hunting and on a phantom using an artificial Hannover vagina (Minitube, Germany). The sperm of each stallion was collected at least three times. The semen quality of 96 stallions of different breeds (Arabian, Akhal-Teke, Trakenen, thoroughbred horse, Oryol trotter, French trotter, Soviet heavy horse, Shetland pony, Welsh pony, horses of different breeds) was analyzed. High variability of sperm quality indicators was revealed. For example, the concentration of sperm in the ejaculate varied from 59 to 514 million /ml, progressive mobility from 20 to 87%, and the number of cells without damage from 21.6 to 95.6%. Such variability of the studied features makes it possible to assume that their formation in the process of ontogenesis occurs under the influence of genetic factors. The GWAS analysis of ejaculate volume, concentration and motility of stallion sperm revealed potential candidate genes associated with these indicators. The associations identified in our study are promising candidates for further identification of the biological mechanisms underlying the quality of stallion sperm.
Supporting agencies: The work was carried out with the financial support of the Ministry of Education and Science, project No. 121052600354-7
About the Author
E. V. Nikitkina
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Ph.D. biological sciences, laboratory of developmental biology
References
1. Nikitkina E. / E. Nikitkina E., K. Plemyashov, A. Musidray, A. Kudinov, A. Krutikova, N. Demetieva // Search for genomic associations with sperm quality of Holstein and Black and White bulls. Genetics and breeding of animals. 2019:4:9-13. (In Russ.) https://doi.org/10.31043/2410-2733-2019-3-3-10
2. Moroz, L.G. Polarographic method for assessing energy metabolism in sperm by stimulating respiration with 2,4-dinitrophenol / L.G. Moroz, I.Sh.. Shapiev // Bulletin of the state scientific institution All-Russian Scientific Research Institute of Genetics and Breeding of Farm Animals. 1978: 33: 28-30.
3. Leeb, T. The horse genome project--sequence based insights into male reproductive mechanisms / T. Leeb // Reprod. Dom. Anim. - 2007. - №42. – C. 45–50. -https://doi.org/10.1111/j.1439-0531.2007.00897.x
4. Beurois, J. CFAP70 mutations lead to male infertility due to severe asthenoteratozoospermia/ J. Beurois; G. Martinez, G.; C. Cazin, C.; Z. Kherraf, Z. E.; A. Amiri-Yekta, N. Thierry-Mieg, M. Bidart, G. Petre, V. Satre, S. Brouillet, A. Touré, C. Arnoult, P. Ray, C. Coutton // A case report. - Hum. Reprod. – 2019. - № 34. – C. 2071–2079. -https://doi.org/10.1093/humrep/dez166
5. Fagerberg, L. Analysis of the human tissue-specific expression by genomewide integration of transcriptomics and antibodybased proteomics/ L. Fagerberg, B. Hallström, P. Oksvold, C. Kampf, D. Djureinovic, J. Odeberg, M. Habuka, S. Tahmasebpoor, A. Danielsson, K. Edlund, A. Asplund, E. Sjöstedt, E. Lundberg, C. Szigyarto, M. Skogs, J. Takanen, H. Berling, H. Tegel, J. Mulder, P. Nilsson, J. Schwenk, C. Lindskog, F. Danielsson, F. Mardinoglu, A. Sivertsson, K. von Feilitzen, M. Forsberg, M. Zwahlen, I. Olsson, S. Navani, M. Huss, J. Nielsen, F. Ponten, M. Uhlén// Mol. Cell. Proteomics. 2014. № 13. – C. 397–406. https://oi.org/10.1074/mcp.M113.035600
6. Liu, J. Functional SNPs of INCENP Affect Semen Quality by Alternative Splicing Mode and Binding Affinity with the Target BtamiR-378 in Chinese Holstein Bulls/ J. Liu, Y. Sun, C. Yang, Y. Zhang, Q. Jiang, J. Huang, Z. Ju, X. Wang, J. Zhong, C.Wang// PloS one. - 2016. - № 11. –C. e0162730. -https://doi.org/10.1371/journal.pone.0162730
7. Leahy, T. Quantitative Proteomic Analysis of Seminal Plasma, Sperm Membrane Proteins, and Seminal Extracellular Vesicles Suggests Vesicular Mechanisms Aid in the Removal and Addition of Proteins to the Ram Sperm Membrane/ T. Leahy, J. Rickard, T. Pini, B. Gadella, S. de Graaf// Proteomics.- 2020. - №20.- C. e1900289. -https://doi.org/10.1002/pmic.201900289
8. Atroshchenko, M.M., Biochemical markers of stallion sperm quality (review) / M.M. Atroshchenko, D.V. Medvedev // Agricultural biology .2023:T.58:2:249-259. doi: 10.15389/agrobiology.2023.2.249rus
9. Huang, M. Involvement of ALF in human spermatogenesis and male infertility/ M. Huang, H. Wang, J. Li, Z. Zhou, Y. Du, M. Lin, J. Sha, J.//Int. J. Mol. Med. – 2006. - № 17. – C. 599–604. - https://doi.org/10.3892/ijmm.17.4.599
10. Yan, W. Expression of the E3 SUMO-1 ligases PIASx and PIAS1 during spermatogenesis in the rat/ W. Yan, H. Santti, O. Jänne, J. Palvimo, J. Toppari // Gene Expr. Patterns. – 2003. - №3. – C. 301–308. - https://doi.org/10.1016/S1567-133X(03)00045-0
11. Suetsugu, S. Male‐specific sterility caused by the loss of CR16/ S. Suetsugu, Y. Banzai, M. Kato, K. Fukami, Y. Kataoka, Y. Takai, N. Yoshida, T. Takenawa //Genes Cells. – 2007.-№ 12. – C. 721–733 - https://doi.org/10.1111/j.1365-2443.2007.01088.x
12. Roy, A. De novo sequencing and comparative analysis of testicular transcriptome from different reproductive phases in freshwater spotted snakehead Channa punctatus / A. Roy, R. Basak, U. Rai // PLoS One. - 2017. - №12. – C. e0173178. -https://doi.org/10.1371/journal.pone.0173178
13. Rodríguez-Marí, A. Sex reversal in zebrafish fancl mutants is caused by Tp53-mediated germ cell apoptosis/ A. Rodríguez-Marí, C. Cañestro, R. BreMiller, A. Nguyen-Johnson, K. Asakawa, K. Kawakami, J. Postlethwait,// PLoS Genet. – 2010.-№6. – С. e1001034. - https://doi.org/10.1371/journal.pgen.1001034
Review
For citations:
Nikitkina E.V. The genetic basis of the sperm quality of stallions. International Journal of Veterinary Medicine. 2023;(4):413-420. (In Russ.) https://doi.org/10.52419/issn2072-2419.2023.4.413
Views:
237
Email this article 