The effect of nonsteroidal and steroid anti-inflammatory drugs on platelet activation IN VITRO

The article presents the results of a study on the effect of steroid and nonsteroidal anti-inflammatory drugs on platelets.

The object of the study was plasma obtained  from  rat  blood  (n=10).  Blood  sampling (2.0 ml from each animal) was carried out  from  the  tail  vein  into  test  tubes  with sodium citrate. Cell elements were counted directly in blood and in platelet plasma using a MEK-6550 hematological analyzer.

The  ability  to  influence  platelet  activation was determined in drugs such as dexamethasone,  ketarol  and  miloxicam,  which were injected into test tubes before plasma reactivation.

 It  was  found  that  the  drugs  of  these groups affect the metabolism of these cells and inhibit their ability to cause clot contraction.  It  was  found  that  the  strength  of  the negative  effect  on  clot  contraction  in  the steroid drug dexamethasone, which through intermediaries  suppresses  the  activity  of phospholipase-A2 and the nonsteroidal drug ketarol,  which  is  the  predominant  inhibitor of cyclooxygenase of the first type, are similar.  It  was  also  found  out  that  the  drug meloxicam, which is a predominant inhibitor of cyclooxygenase of the second type, causes only a slight decrease in platelet clot contraction. The results obtained in the experiment indicate  a  direct  positive  relationship  between the degree of clot contraction and the amount  of  thromboxane-A2,  which  is  synthesized and released by activated platelets. It  is  known  that  the  ability  of  platelets  to accelerate and enhance regenerative processes is also directly related to their activation. Based  on  this,  it  is  suggested  that  patients who have been  prescribed  plateletenriched plasma  therapy  should  not  use  glucocorticoids and preparations with a high degree of selectivity  to  cyclooxygenase  of  the  first type as anti-inflammatory drugs.

1. The influence of leukocyte admixture on the wound-healing effect of a platelet clot / A.V. Bokarev, M.V. Sverdlova, A.O. Minina, R.D. Kholodny // International Bulletin of Veterinary Medicine. – 2022. – No. 4. – pp. 427-433.

2. The influence of platelet-derived growth factors on cell proliferation / A.N. Sveshnikova, E.V. Shamova, I.S. Kolesnikova [et al.] // In the book: Collection of theses of the XXIV Congress of the I. P. Pavlov Physiological Society. Collection of theses of the Congress. St. Petersburg, 2023. – p. 223.

3. The reaction of the skin of rats to the introduction of autologous cell-free blood plasma or plasma containing platelets and other leukocytes / M.V. Sverdlova, A.V. Bokarev, A.A. Stekolnikov, A.O. Minina // Issues of regulatory regulation in veterinary medicine. - 2021. – No. 3. – pp. 92-93.

4. Bazikyan, E.A. comparative analysis of the use in clinical practice of a fibrin clot enriched with leukocytes and platelets when filling holes of extracted teeth / E.A. Bazikyan, I.I. Tarba, G.A. Volozhin // Russian dentistry. – 2020. – Vol. 13. – No. 1. – pp. 16-17.

5. On the issue of the use of platelet-enriched fibrin clots / S.G. Pakhlevanyan, L.V. Shevchenko, A.Yu. Shevchenko [et al.] // Actual problems of medicine. – 2022. – Vol. 45. – No. 4. – pp. 388-399.

6. Experience of using platelet-enriched plasma in the treatment of patients with extensive wound defects / D.S. Tretyak, A.P. Trukhan, D.V. Vasiliev [et al.] // Medical Journal. – 2023. – № 2 (84). – Pp. 108-112.

7. Stimulation of osteoregeneration with PRP therapy / I.A. Rodin, I.G. Kiselev, L.P. Vishnivetskaya [et al.] // Izvestiya Orenburg State Agrarian University. – 2018. – № 3 (71). – Pp. 186-190.

8. The effect of 3"'-O-acetylembinin on platelet activation induced by thrombin / Pronin N.A. // In the collection: Young pharmacy - the potential of the future. Collection of materials of the XII All-Russian scientific conference of students and postgraduates with international participation. St. Petersburg, 2022. – pp. 397-401.

9. Dynamics of platelet functional responses in the process of thrombin generation during blood clotting: research report (conclusion) : 05-104 [Electronic resource]/ FGBONE CTPFHF RNF ; hand. E.M. Koltsova. – Moscow, 2020. -No. GR 20-75-00057. – URL: https://elibrary.ru/download/elibrary_39266958_81005685.pdf (accessed 13.10.2023).

10. The influence of local antiseptics and anti-inflammatory agents on the functional activity of platelet-rich plasma in the complex therapy of periodontitis / L.Yu. Orekhova, T.V. Kudryavtseva, T.V. Vavilova, V.V. Tachalov // Periodontology. – 2008. – № 1 (46). – Pp. 61-64.

11. Kuznik, B.I. To an objective assessment of the true retraction of a blood clot / B.I. Kuznik, M.A. Kotovshchikova // Laboratory business. – 1964. – No. 9. – p. 524.

12. Metabolic processes and mechanisms of regulation of platelet activity (literature review) /A.V. Khaliulin, O.A. Gusyakova, A.V. Kozlov, A.I. Gabrilchak // Clinical laboratory diagnostics. – 2019. – No. 3. – pp. 164-169.

13. Morphology of fibrin and fibrin-platelet & fibrin-platelet-leukocyte clots / M. Sverdlova, A. Bokarev, A. Stekolnikov [et al.] // International Transaction Journal of Engineering, Management and Applied Sciences and Technologies. - 2021. – Vol. 12. – No. 7. – P. 12A7N.

14. Shpakova, V.S. The effect of arachidonic acid on platelet functions / V.S. Shpakova, S.P. Gambaryan, N.I. Hiltkina // In the collection: receptors and intracellular signaling / [Edited by V.P. Zinchenko, A.V. Berezhnov] - Receptors and intracellular signaling. – 2019. – pp. 123-127.

15. A method for obtaining platelet-enriched plasma from small volumes of blood [Text]: pat. 2789518 Grew. Federation: IPC A 61 K 35/19, A 61 K 35/16, G 01 N 33/49 / A.A. Stekolnikov, A.V. Bokarev, V.E. Gorokhov [et al.]; applicant and patent holder of the SPbGUVM. - No. 2022113042; application 13.05.2022; publ. 06.02.2023, Bul. No. 4. – 5 pages: ill.