Toxicity of the feed additive when using the Tetrahymena Pyriformis test object

Abstract

To compensate for the lack of minerals in the feed, livestock breeders additionally use various sources of macro- and microelements, including copper-containing components. The aim of our research work was the use of an in vitro method for determining the toxicity of feed additives using the test object Tetrahymena pyriformis. Biotesting was carried out at the Research Institute PVMiB UO VGAVM guided by GOST 13496.7-97, GOST 31674-2012 and "Methodological recommendations for the accelerated determination of the toxicity and safety of feed and feed additives using the test object Tetrahymena pyriformis". Before starting the assessment of general toxicity, we conducted a study of the basic feed premix for broiler chickens. Each sample was studied 3 times.
After receiving the results, various coppercontaining additives were added to the compound feed at the rate of 2.0%. Then we repeated a threefold toxicological examination. To obtain objective empirical data, each test sample of copper-containing additives (name and manufacturer) was encrypted by us.We have conducted studies on the simplest five feed additives at the rate of 2.0%. Based on in vitro laboratory tests, it was found that the death of protozoa, as well as a change in the shape and nature of the movement of Tetrahymena pyriformis, in the five samples presented, were at the level of 46.2-30.5%, which indicates an average degree of toxicity of copper-containing additives. Copper-containing feed additives can be used for further research work, in particular, in diets for laboratory and farm animals, taking into account their strict rationing in the diet.