Determination of antimicrobial activity of a phytosorption complex in vitro

Abstract

A large number of tests have already proved that it is natural components, or rather their complexes, that can adequately replace most existing antimicrobial agents. So, at the Department of Pharmacology and Toxicology FSBEI SPbGAVM developed a whole line of antimicrobial and antimycotic drugs based on plant and sorption components. In this experiment, the minimum concentrations of phytosorbton complexes with antimicrobial activity in vitro were determined. For this, the well method was used in relation to reference strains of microorganisms. The studies were carried out according to standard methods for the following microorganisms: Klebsiella pneumoniae, Enterococcus faecalis, Enterobacter cloacae, Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacterbaumannii. Three types of phytosorption complex were used as material for the study. Compound № 1, 2, and 3 included sorbents with already proven antimycotic activity. In addition to sorbents, oregano oil and thyme herb containing carvacrol were added to sample № 1, a preparation representing the amount of bisulfates of natural alkaloids sanguinarine and heleritrin was added to compound № 2, and sample № 3 - all three components - oregano oil, thyme grass, and a source of natural alkaloids sanguinarine and hel-erythrin. Based on the data obtained, it can be concluded that compound № 3, which includes both sorbents and oregano oil, thyme herb and a source of natural alkaloids sanguinarine and chelerythrin, has a high antimicrobial effect. This complex should be considered as a possible alternative to antibiotics, taking into account the receipt of safe and quality products.