Calculation of reference intervals for indicators of thyroid hormones and cortisol in horses
https://doi.org/10.52419/issn2072-2419.2024.1.287
Abstract
The article discusses the results of calculating reference intervals for thyroid hor mones and cortisol in adult horses. The study included the results of a laboratory study of the concentrations of total triiodothyronine, total and free thyroxine and cortisol from 50 horses aged 3 to 29 years, including 17 mares, 13 stallions and 20 geldings. All animals underwent an extensive biochemical blood test, the results of which revealed no pathological changes.
To calculate the normative limits of fluctuations for each hormone, calculations were carried out using the Tukey method, which is used to determine the reference limits in the case of a small number of variants in the group and the absence of a normal distribution. Microsoft Excel was used for calculations. The conducted studies made it possible to clarify the reference intervals for thyroid hormones and cortisol in horses, which were: total triiodothyronine (T3) - 1.13-4.04 nmol/l, total thyroxine (T4) - 20.97-57.42 nmol/l, free thyroxine (fT4) 7.38-23.07 pmol/l, cortisol – 39.42-227.02 nmol/l. The results obtained can be recommended for use not only for the clinical and biochemical laboratory of St. Petersburg State University of Medicine, but also for all veterinary laboratories using diagnostic test systems for enzyme immunoassay of Vital Development Corporation.
About the Authors
S. V. Vasileva
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Candidate of Veterinary Sciences, Associate Professor of the Department of Biochemistry and Physiology
L. Yu. Karpenko
St. Petersburg State University of Veterinary Medicine
Russian Federation
Russian Federation
Doctor of Biological Sciences, Prof., Head. department Biochemistry and Physiology
References
1. Equine Clinical Pathology / Edited by Raquel M. Walton. – John Wiley & Sons, 2013. – 296 р.
2. Fey, K. Equine Cushing syndrome (ECS). Case report, review of its diagnosis and therapy and substantial differences from Cushing syndrome in dogs / K. Fey, E. Jonigkeit, A. Moritz // Tierarztliche Praxis. Ausgabe G, Grosstiere / Nutztiere. –1998. – Vol. 26, №1. – P. 41-47.
3. Frank, N. Equine thyroid dysfunction / N. Frank, J. Sojka, N. T. Messer // The Veterinary clinics of North America. Equine practice. – 2002. – Vol. 18, №2. – P.305-319, vii. doi:10.1016/s0749-0739(02)00007-x
4. Love, S. Equine Cushing's disease / S. Love // The British veterinary journal. – 1993. – Vol. 149, №2. – P. 139-153. doi: 10.1016/S0007-1935(05)80084-3
5. Breuhaus, B. A. Disorders of the equine thyroid gland / B. A. Breuhaus, // The Veterinary clinics of North America. Equine practice. –2011. – Vol. 27, №1. – P. 115-128. doi: 10.1016/j.cveq.2010.12.002
6. Tangyuenyong, S. Sensitive radioimmunoassay of total thyroxine (T4) in horses using a simple extraction method / S. Tangyuenyong, Ya. Nambo, K. Nagaoka [et al.] // Journal of Veterinary Medical Science. – 2017. – Vol. 79, № 7. – P. 1294-1300.
7. Beech, J. Evaluation of Thyroid, Adrenal, and Pituitary Function / J. Beech // Veterinanj Clinics of North America: Equine Practice. – 1987. –Vol. 3, No 3. – P. 649-660.
8. McCue, Patrick M. Equine Cushing's disease / Patrick M. McCue // The Veterinary clinics of North America. Equine practice. – 2002. – Vol. 18, №3. – P. 533-543, viii. doi: 10.1016/s0749-0739(02)00038-x
9. Rendle, D. I. Investigation of single and paired measurements of adrenocorticotropic hormone for the diagnosis of pituitary pars intermedia dysfunction in horses / D. I. Rendle, M. Duz, J. Beech, T. Parkin, A. E. Durham // Journal of veterinary internal medicine. – 2015. – Vol. 29, №1. – P. 355-361. doi: 10.1111/jvim.12489
10. Keen, J. A. Biochemical indices of vascular function, glucose metabolism and oxidative stress in horses with equine Cushing's disease / J. A. Keen, M McLaren, K J Chandler, B C McGorum // Equine veterinary journal. –2004. – Vol. 36, №3. – P. 226-229. doi: 10.2746/0425164044877215
11. Vasilyeva, S.V. Study of the concentration of thyroxine and cortisol in cows with fatty hepatosis during the transit period / S.V. Vasilyeva, L.Yu. Karpenko // Issues of legal regulation in veterinary medicine. – 2019. – No. 3. – P. 202-204. – DOI 10.17238/issn2072-6023.2019.3.202.
12. Vasilyeva, S.V. Study of thyroid status in fresh cows with liver dysfunction / S.V. Vasilyeva, L.Yu. Karpenko // Issues of legal regulation in veterinary medicine. – 2021. – No. 4. – P. 137-140. – DOI 10.52419/issn2072-6023.2021.4.137.
13. Stekolnikov, A. A. Analysis of the main statistical parameters when studying the dynamics of thyroid hormones in cows of a productive herd / A. A. Stekolnikov, S. V. Vasilyeva, M. A. Ladanova // Issues of legal regulation in veterinary medicine. – 2015. – No. 3. – P. 238-240.
14. The effect of Mepron®, lipoic acid and L -carnitine on thyroid function in cows during the transit period / S. Vasileva, L. Karpenko, R. Vasilev [et al.] // Reproduction in Domestic Animals. – 2019. – Vol. 54, No. S3. – P. 97.
15. Study of metabolic processes in cows with hyperbilirubinemia in the postpartum period / A. Nikitina, S. Vasileva, R. Vasilev [et al.] // FASEB Journal. – 2022. – Vol. 36, No. S1. – P. 3431. – DOI 10.1096/fasebj.2022.36.S1.R3431. – EDN VDGVPC.
16. Sojka, J.E. Serum triiodothyronine, total thyroxine, and free thyroxine concentrations in horses / J.E. Sojka, M.A. Johnson, G.D. Bottoms // Am J Vet Res. –1993. – Vol. 54, №1. – P. 52-55.
17. Kaneko J.J. Clinical biochemistry of domestic animals / J.J. Kaneko, J.W. Harvey, M.L. Bruss – 6-th ed. 2008/ - 916 p.
18. Karpenko, L. Yu. Dynamics of the content of thyroid hormones in the blood serum of horses in connection with the body’s supply of iodine and selenium / L. Yu. Karpenko, R. N. Selimov, A. A. Bakhta // Scientific notes of the Kazan State Veterinary Academy medicine named after N.E. Bauman. – 2010. – T. 203. – P. 118-122.
19. Larsson, M. Plasma cortisol in the horse, diurnal rhythm and effects of exogenous acth / M. Larsson, LE. Edqvist, L. Ekman, et al. // Acta Vet Scand. – 1979. – Vol. 20, P.16–24.
20. Katayev, A. Establishing reference intervals for clinical laboratory test results. Is there a better way? / A. Katayev [et al.] // Am. J. Clin. Pathol. – 2010 – № 133 – Р. 180–186.
21. Arkhipkin, A. A. Methodology for calculating reference intervals of laboratory parameters using the example of fetal proteins / A. A. Arkhipkin, O. V. Lyang, A. G. Kochetov // Bulletin of the Russian Military Medical Academy. – 2014. – No. 3(47). – pp. 23-25.
Review
For citations:
Vasileva S.V., Karpenko L.Yu. Calculation of reference intervals for indicators of thyroid hormones and cortisol in horses. International Journal of Veterinary Medicine. 2024;(1):287-294. (In Russ.) https://doi.org/10.52419/issn2072-2419.2024.1.287
Views:
219
Email this article 