Study of comparative therapeutic and prophylactic efficacy of fluoroquinolone antibiotics in experimental staphylococcosis of day-old chicks

Staphylococcosis in chicks raises much concern among poultry industry specialists and improving methods of its containing is still an urgent problem. We conducted a comparative study as for therapeutic and prophylactic efficacy of several fluroquinolone drugs in chicks, experimentally infected with Staphylococcus aureus. The chicks were challenged intraperitoneally with two different field isolates of S. aureus in suspension. One of the two isolates was resistant to fluoroquinolones, the other was susceptible to them. The chicks began receiving the drugs via drinking water ad libitum at 48 h prior to the challenge, at a concentration 200 mg/L. The treatment lasted 5 days. The results of our study show that the highest therapeutic effectiveness (96% and 88%) was achieved in the groups, which had been challenged with an S. aureus strain, susceptible to fluoroquinoloes and treated with enrofloxacin and levofloxacin, respectively. In the group, which had been challenged with a susceptible strain and treated with ciprofloxacin, therapeutic effectiveness was as just high as 68%. The results of our study also show rather low effectiveness of ofloxacin p.o. for treatment of staphylococcosis in chicks. When the chicks had been challenged with an S. aureus strain, resistant to fluoroquinolones, the mortality rate was as high as 96%. When they had been challenged with an S. aureus strain, susceptible to ofloxacin, the mortality rate of the poultry was rather high too - 52%. For most of the chicks in this group the infection became chronic, making the prospects of their treatment and subsequent use in the farm obscure. Administration of norfloxacin proved its low effectiveness, very much like ofloxacin. For the two groups, receiving norfloxacin, the mortality rate among the challenged chicks was as high as 80% and 76%, respectively.

1. Bakulin V.A. Bolezni ptits [Diseases of birds] / V.A. Bakulin // Saint Petersburg, 2006, 688 p. (In Russ.)

2. Balbutskaya A.A. Chuvstvitel’nost’ k antibakterial’nym sredstvam vozbuditelei klinicheskogo mastita korov [Antimicrobial resistance of isolates causing clinical mastitis in cows] / A.A. Balbutskaya, V.N. Skvortsov, S.S. Belimova // Veterinariia [Veterinariya], 2018, no. 9, pp. 39-44. DOI: 10.30896/0042-4846.2018.21.9.39-44. (In Russ.)

3. Zaharova L.N. Eksperimental’naia stafilokokkovaia infektsiya: mikrobiologicheskiye i immunomorfologicheskiye aspekty [Experimental staphylococcal infection: microbiological and immunological aspects] / L.N. Zaharova, A.K. Baltabaeva, Yu.A. Pimenova, T.A. Ageeva, A.N. Evstropov // Sibirskoie meditsinskoie obozreniie [Siberian Medical Review], 2010, no. 4, pp. 46-49. (In Russ.)

4. Bolezni domashnikh i sel’skokhoziaistvennykh ptits. Per. s angl. Chast’ 3 / B.U.Kelnek, Kh.Dzh.Barns, Ch.U.Bierd i dr. [Diseases of poultry. Edited by Bruce W. Calnek, H. John Barnes, Charles W. Beard et al., Vol. 3, translated from English], Moscow, Aquarium Publ., 1997, 1232 p. (In Russ.)

5. Yurin D.V. Antimikrobnaia aktivnost’ ftorkhinolonov v otnoshenii mikroorganizmov, vydelennykh ot zhivotnykh [Antimicrobial activity of fluoroquinolones concerning the microorganisms allocated from animals] / D.V. Yurin, A.A. Balbutskaya, V.N. Skvortsov, A.A. Prisnyi // Mezhdunarodnyi vestnik veterinarii [International Bulletin of Veterinary Medicine], 2018, no. 3, pp. 63-67. (In Russ.)

6. Yurin D.V. Izuchenie perenosimosti enrofloksatsina tsypliatami i ego ispol’zovanie pri lechenii eksperimetal’nogo sal’monellioza [Study of tolerance to enrofloxacin in chickens and employment of enrofloxacin for treatment of experimental salmonellosis] / D.V. Yurin et al. // Aktual’nye voprosy sel’skokhoziaistvennoi biologii [Actual issues in agricultural biology], 2020, no. 4 (18), pp. 46-52. (In Russ.)

7. Yurin D.V. Antimikrobnaia aktivnost’ enrofloksatsina in vitro [Antimicrobial activity of enrofloxacin in vitro] / D.V. Yurin, V.V. Nevzorova, A.A. Balbutskaya, S.S. Belimova // Mezhdunarodnyi vestnik veterinarii [International Bulletin of Veterinary Medicine], 2020, no. 2, pp. 99-103. DOI: 10.17238/issn2072-2419.2020.2.31. (In Russ.)

8. John, J Jr. The treatment of resistant staphylococcal infections / J Jr. John// F1000Res. – 2020. – №26. – Р. 213-221.

9. Kizerwetter-Świda, M. Genetic characterization of coagulase-positive staphylococci isolated from healthy pigeons / M. Kizerwetter-Świda, D. Chrobak-Chmiel, M. Rzewuska, A. Antosiewicz, B. Dolka, A. Ledwoń, A. Czujkowska, M. Binek// Pol. J. Veter. Sci. – 2015. – № 18. – Р 627-634.

10. McNamee, P.T. Bacterial chondronecrosis with osteomyelitis (’femoral head necrosis’) of broiler chickens: A review / P.T. McNamee, J.A. Smyth// Avian Pathol. – 2000. – № 29. – Р. 253-270.

11. McNamee, P.T. Study of leg weakness in two commercial broiler flocks / P.T. McNamee, J.J. Mccullagh, B.H. Thorp at all// Veter Rec. – 1998. – № 143. – Р. 131-135.

12. Peton, V. Staphylococcus aureus in veterinary medicine / V. Peton// Infect.Genet. Evol. – 2014. – № 21. – Р. 602-615.

13. Swayne, D.E. Diseases of Poultry / D.E. Swayne. – Hoboken: John Wiley & Sons, 2020. – рр. 995-1003.

14. Szafraniec, G.M. Review of Current Knowledge on Staphylococcus agnetis in Poultry / G.M Szafraniec, Szeleszczuk P, Dolka B. A //. – Animals (Basel). – 2020. – № 10. – Р. 1421.