The effect of the drug "prostimul" on the natural resistance and gene expression level of piglets with hypotrophy

The problem of the birth of piglets with hypotrophy in the conditions of modern industrial pig farming causes great economic damage to this industry. Various forms of immunodeficiency are of great importance in the pathogenesis of hypotrophy. In this regard, an active search is underway for effective drugs for veterinary use to combat this pathology. One of these drugs is "Prostimul", which includes a signal peptide of the first type belonging to the cytokine group, as well as vitamins A, E, C. This article describes studies conducted on a group of hypotrophic piglets in order to determine the effect of the drug on immunological blood parameters, namely, an assessment of weasels, helmets, general IG, CEC, CT, SP, ETC. "Prostimuli" were administered twice to a group of hypotrophic piglets, on the first and third days of life in a dose 0.1 ml/kg of body weight. Blood samples for the study were taken on the 1st, 7th, 14th and 21st days. According to the results of the experiment, it was found that the double use of the drug "Stimul" contributed to the normalization of immune functions, which was already visible on the 7th day of the experiment. Thus, in hypotrophic piglets of the experimental group and normotrophics, the indicators of BASK, WEASEL, CEC and general IG were identical. In piglets with hypotrophy treated with the drug "Prostimul", there was a significant increase in the level of Ifn-α expression by days 7 and 14 – by 56.73 times compared with normotrophics. By day 14, an increase in the level of Tgf-β expression was also noted, indicating activation of immune processes in piglets with hypotrophy. Interestingly, by day 21, there was a decrease in the level of Ifn-α expression by 5.33 times compared with normotrophics after using the drug "Prostimul", which may indicate a decrease in inflammatory processes. Two-time use of the drug, in our opinion, should be considered optimal due to its stable effect throughout the experiment.

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