Post-vaccinal diagnostics of the immune response induced by whole-virion, recombinant and subunit vaccines against highly pathogenic avian influenza
https://doi.org/10.52419/issn2072-2419.2024.4.14
Abstract
The article presents the results of a study of the immune response of chickens of the Dominant cross to vaccination with domestic and foreign vaccines against highly pathogenic avian influenza subtype H5N1. The following drugs were used as vaccines: Flu Protect H5, Reassortant Trivalent Vaccine (H5N6(H5Re13)+H5N8(H5Re14)+H7N9 (H7Re4)" and "Volvac® B.E.S.T. AI+ND". For quantitative analysis of the antigenic activity of vaccines in blood sera, ID Screen® Influenza H5 Indirect kits were used, designed to determine the titer of antibodies to hemagglutinin H5, and ID Screen® Influenza A Antibody Competition Multispecies, allowing to measure the titer of antibodies to NP protein (nucleoprotein of the influenza A virus). According to the results of the study, the Reassortant Trivalent Vaccine demonstrated the highest average antibody titer among the tested samples (10709.5), which indicates its high immunogenicity. The lowest efficiency indicators were shown by the vaccine "Volvac® B.E.S.T. AI+ND" with an average antibody titer of 2627.5. All three vaccines demonstrated high protective activity against influenza A virus of the H5N1 subtype, but the effectiveness and resistance of the immune response varied. A parallel study of blood sera using test kits focused on various influenza virus antigens (hemagglutinin and nucleoprotein) showed the possibility of differentiating an infected bird from a vaccinated one. This approach proved to be particularly effective in the use of subunit vaccines, allowing us to reliably distinguish natural infection from the vaccine immune response.
About the Authors
N. V. Tarlavin
FGBOU VO SPbGUVM
Russian Federation
Russian Federation
Tarlavin N.V. – Ph.D., Assistant of the Department of Epizootology named after V.P. Urban
V. V. Veretennikov
FGBOU VO SPbGUVM
Russian Federation
Russian Federation
Veretennikov V.V. – Assistant of the Department of Epizootology named after V.P. Urban
E. J. Javadov
FGBOU VO SPbGUVM
Russian Federation
Russian Federation
Javadov E.J. – Doctor of Medical Sciences, Professor of the Department of Epizootology named after V.P. Urban
K. S. Kudrya
Smorodintsev Federal State Budgetary Research Institute of Influenza
Russian Federation
Russian Federation
Kudrya K.S. – laboratory assistant researcher at the
D. A. Kraskov
FGBOU VO SPbGUVM
Russian Federation
Russian Federation
Kraskov D.A. – postgraduate student of the Department of Epizootology named after V.P. Urban
A. V. Degtyareva
FGBOU VO SPbGUVM
Russian Federation
Russian Federation
Degtyareva A.V. – 5th year student of the Faculty of Veterinary Medicine of the
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Review
For citations:
Tarlavin N.V., Veretennikov V.V., Javadov E.J., Kudrya K.S., Kraskov D.A., Degtyareva A.V. Post-vaccinal diagnostics of the immune response induced by whole-virion, recombinant and subunit vaccines against highly pathogenic avian influenza. International Journal of Veterinary Medicine. 2024;(4):14-19. (In Russ.) https://doi.org/10.52419/issn2072-2419.2024.4.14
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