Control of viability and stability of biological properties of the refeence Brucella canis RM 6/66 strain during long-term storage
https://doi.org/10.52419/issn2072-2419.2024.4.35
Abstract
Brucellosis is a particularly dangerous infectious disease that causes enormous economic damage to livestock farms specializing in the breeding of cattle, sheep and pigs. Preservation of pathogens in a viable state with their original properties over a long period is one of the main tasks of the State collections of microorganisms. The aim of this paper was to study the reference strain Brucella canis RM 6/66, stored for more than 20 years in lyophilized form, determining its viability and investigating its biological properties and taxonomic affiliation. The obtained results are showed that the studied strain, when seeded on nutrient media corresponding to this species of pathogen, is viable. The study of its biological properties, using tests to determine dissociation, showed that the strain was dissociated, due to which a selection and cultivation of colonies according to White-Wilson. The pure culture of the strain of Brucеlla canis RM 6/66 in the R-form was obtained, (colonies are dark purple, with smooth edges and an uneven surface). The use of the RT-PCR identification method using species-specific primers and a TagMan probe showed that the strain B. canis RM 6/66 corresponds to the reference strain presented in international databases at all ten loci.
Supporting agencies: The materials were prepared using the funds of the Federal Center for Toxicological, Radiation and Biological Safety within the research “Study of the biological characteristics of strains of infectious and especially dangerous animal diseases and their stability during long-term storage”.
About the Authors
E. V. Pankova
Federal State Budgetary Scientific Institution «Federal Center for Toxicological, Radiation and Biological safety»
Russian Federation
Russian Federation
Pankova E.V. – Candidate of Biological Sciences, Leading Researcher
E. A. Artemeva
Federal State Budgetary Scientific Institution «Federal Center for Toxicological, Radiation and Biological safety»
Russian Federation
Russian Federation
Artemeva E.A. – Candidate of Veterinary Sciences., Senior Researcher, Head of Department – State Collection of Microorganisms
L. A. Melnikova
Federal State Budgetary Scientific Institution «Federal Center for Toxicological, Radiation and Biological safety»
Russian Federation
Russian Federation
Melnikova L.A. - Candidate of Veterinary Sciences., Leading Researcher
E. N. Mustafina
Federal State Budgetary Scientific Institution «Federal Center for Toxicological, Radiation and Biological safety»
Russian Federation
Russian Federation
Mustafina E.N. - Candidate of Veterinary Sciences., Leading Researcher
E. A. Gromova
Federal State Budgetary Scientific Institution «Federal Center for Toxicological, Radiation and Biological safety»
Russian Federation
Russian Federation
Gromova E.A. - Candidate of Biological Sciences, Senior Researcher
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Review
For citations:
Pankova E.V., Artemeva E.A., Melnikova L.A., Mustafina E.N., Gromova E.A. Control of viability and stability of biological properties of the refeence Brucella canis RM 6/66 strain during long-term storage. International Journal of Veterinary Medicine. 2024;(4):35-42. (In Russ.) https://doi.org/10.52419/issn2072-2419.2024.4.35
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