The effect of Viferon-B and Enrofloxavetferon-B on the microbiota of utero-vaginal secretions of cows with endometritis

Endometritis is one of the most frequently detected diseases of cattle, which is associated with disturbances in the microbial balance of the uterine cavity. This pathological process leads to disturbances in animal fertility, and, consequently, to economic losses in the dairy industry. In this regard, it is important to carry out timely preventive measures and have effective drugs for the treatment of postpartum endometritis. The aim of this study was to evaluate the effect of drugs based on bovine recombinant interferon "Biferon-B" and "EnrofloxacinferonB" on the taxonomic composition of bacteria in the uterine-vaginal discharge of patients with acute postpartum catarrhal endometritis of cows using high-throughput sequencing. It was found that the dominant species in the uterine cavity of cows with endometritis were Porphyromonas levii, Bacteroides heparinolyticus and Fusobacterium necrophorum. Bacterial taxa capable of causing various cattle diseases were detected in uterovaginal secretions. It was found that therapy of cows with the drug "Biferon-B" after 24 hours led to a tendency to increase the number of Caviibacter abscessus, Mycoplasmopsis californica and Histophilus somni. Statistically significant differences were found in the number of Porphyromonas SGB1983 before therapy (0.9%) and after therapy with "Enrofloxacinferon-B" during the first 24 hours (9.6%) (p<0.05). The study of the microbial diversity of utero-vaginal secretions of cows during therapy with "Enrofloxacinferon-B" on the seventh day established a tendency to decrease the relative abundance of bacteria Bacteroides heparinolyticus, Trueperella pyogenes and Mycoplasmopsis californica. The obtained data expand our understanding of the etiology of acute postpartum catarrhal endometritis in cows and the effect of drugs based on species-specific interferons on the course of this disease.

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