The effect of the drug "Stimul" on the bone marrow hematopoiesis of piglets with prenatal hypotrophy
https://doi.org/10.52419/issn2072-2419.2024.4.92
Abstract
The article presents the results of studies evaluating the effect of the drug "Prostimul" on bone marrow hematopoiesis of piglets with perinatal hypotrophy. The experiment was conducted in a large pig farm, where newborn hypotrophic piglets were twice administered the drug "Prostimul" at a dose of 0.1 ml / kg of body weight on the 1st and 3rd days of life and changes in the bone marrow were evaluated. Background examination of the bone marrow in hypotrophic piglets showed the presence of erythroblasts and proerythrocytes and an increase in immature myeloid cells, which are an indicator of the initial stage of the inflammatory process. When analyzing the myelogram, the ratio of leukocytes to erythrocytes in normotrophic piglets turned out to be 1.4 times (p <0.05) higher than in hypotrophics, and the index of erythrocaryocyte maturation in hypotrophic piglets was 21.3% higher (p <0.05) than in normotrophic piglets. After using the drug "Prostimul", hypotrophic piglets on day 7 showed an increase in the number of polychromatophilic erythrocytes, a significant increase in the number of rodshaped and segmented neutrophils. The bone marrow index of neutrophils characterizing the activity of myelopoiesis in hypotrophic piglets after the use of the drug "Prostimul"was 8.8% higher than in normotrophic piglets. Two-time administration of the drug "Prostimul"to piglets with perinatal hypotrophy at a dose of 0.1 ml / kg of body weight had a positive effect on erythroid and myeloid sprouts, stimulating erythropoiesis and myelopoiesis. These changes indicate a positive effect of the drug on the compensatory mechanisms of hematopoiesis, which leads to an improvement in the overall health of piglets and a reduction in the risk of infections.
Supporting agencies: "The research was carried out at the expense of a grant from the Russian Science Foundation No. 23-26- 00020, https://rscf.ru/project/23-26- 00020 /".
About the Author
E. V. Mikhailov
All-Russian Veterinary Research Institute of Pathology, Pharmacology and Therapy (ARVRIPP&T)
Russian Federation
Russian Federation
Mikhailov E. V. – Candidate of Veterinary Sciences, Senior researcher, Head of the Department of Experimental Pharmacology and Living Systems Functioning
References
1. Mandro N.M. Bone marrow, its composition and structure in comparison in wild ruminants and farm animals / N.M. Mandro, T.V. Fedorenko // Bulletin of the Krasnoyarsk State Agrarian University. - 2013. – No. 5. – pp. 181-184.
2. Shabunin B.V. Morphofunctional state of the immune system organs in hypotrophic piglets in the early neonatal period / B.V. Shabunin, A.V. Nekrasov, E.M. Stepanov, [et al.] // Agrarian Bulletin of the Urals. – 2024. – p. 932.
3. Izak G. “Erythroid cell differentiation and maturation / G. Izak // Progress in hematology. – 1977. - No. 10. - p. 1-41.
4. Kagu M. B. et al. Bone marrow macrophage iron stores in patients with HIV infection and AIDS-associated Kaposi's sarcoma / M. B. Kagu et al. // African journal of medicine and medical sciences. -2007. - No. 36. – pp. 125-128.
5. Paulson R.F., Lei Shi, Dai-chen Wu. Stress erythropoiesis: new signals and new stress progenitor cells/ R. F. Paulson., Shi Lei, Wu. Dai-chen // NIH Public Access Author Manuscript Curr Opin Hematol. – 2011. – No.18. – pp.139–145.
6. Popov V.S. Secondary immunodeficiency in pigs: clinical and immunological characteristics and principles of immunocorrection / V.S. Popov, N.V. Samburov, N.V. Vorobyova, [et al.] // Bulletin of the Kursk State Agricultural Academy – 2016. – No. 3. – pp. 57-61.
7. Ksenzova T.I. and others. The role of immunophenotyping of bone marrow and cerebrospinal fluid cells in the diagnosis of multiple myeloma and plasma cell leukemia / T. I. Ksenzova, E.V. Anikina, O.V. Ananyeva, [et al.] // Fundamental Research. – 2012. – No. 8-1. – pp. 101-106.
8. Tatoyan M. Ontogenesis of the pig erythroid cells / M. Tatoyan, E. Karalova, L. Hakobyan [et al.] // Porcine Research. – 2015. – Vol. 5. – No. 1. – pp. 12-22.
9. Khasanov B.B. Hematological features in toxic hepatitis / B.B. Khasanov // Achievements of science and education. – 2022. – №. 5 (85). – Pp. 53-59.
10. Abrashova T.V. Handbook. Physiological, biochemical and biometric indicators of the norm of experimental animals. St. Petersburg. / T.V. Abrashova, Ya.A. Gushchin., M.A. Kovaleva, [et al.] // Publishing house "LEMA", 2013. – 116 p.
11. Rukavishnikova S.A. Fundamentals of clinical immunology / S. A. Rukavishnikova., A.S. Pushkin, T.A. Akhmedov // – 2015.
12. Kazyuchits M.V. Influence of sodium thiosulfate and vitamin C on biochemical blood parameters and myelogram in piglets during immunization with the ATP vaccine / M.V. Kazyuchits // – 2011.
13. Bulgakova O.S. Changes in the morphological composition of blood under stress and as a result of corrective sessions using the biofeedback method / O.S. Bulgakova // Psychology of education in a multicultural space. - 2010. – Vol. 1. – No. 1. – pp. 102-115.
14. Detivaud L. Hepcidin levels in humans are correlated with hepatic iron stores, hemoglobin levels, and hepatic function / L. Detivaud, E. Nemeth, K. Boudjema [et al.] // Blood. – 2005. – Vol. 106. – No. 2. – pp. 746-748.
15. Saroyan D.A. Violation of myelopoiesis in the acute form of African swine fever: dissertation of the National Academy of Sciences of the Republic of Armenia Institute of Molecular Biology, 2016.
16. Siraziev R.Z. Hypotrophy of piglets in industrial pig farming / R.Z. Siraziev // Bulletin of the Buryat State Agricultural Academy named after V.R. Filippov. 2021. No. 4 (65). pp. 84-94.
17. Mikhailov E. PSVIII-6 Cytomorphological features of the bone marrow of hypotrophic piglets / E. Mikhailov, Y. Shaposhnikova, B. Shabunin [et al.] // Journal of Animal Science. – 2020. – Vol. 98. – no. Supplement_4. – pp. 256-256.
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For citations:
Mikhailov E.V. The effect of the drug "Stimul" on the bone marrow hematopoiesis of piglets with prenatal hypotrophy. International Journal of Veterinary Medicine. 2024;(4):92-99. (In Russ.) https://doi.org/10.52419/issn2072-2419.2024.4.92
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