SENSITIVITY OF BACTERIA TO ANTIBIOTICS MASTI BLOCK® DC 49, MASTIC-P, MASTERCARD WITH MASTITIS OF DRY COWS
https://doi.org/10.52419/issn2072-2419.2024.4.161
Abstract
In the etiology of mastitis, pathogenic bacteria Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Corynebacterium bovis and a number of others are of paramount importance. Frequent unjustified use of antibacterial drugs without prior determination of effectiveness against microorganisms in a particular farm led to the formation of resistant isolates. The aim of the research was to study the sensitivity of bacterial strains isolated from cows with mastitis to the antibacterial drugs MASTIBLOCK® DC 49, MASTICAN-P and MASTIGARD. The research was carried out in a large dairy complex in the Central district of the Russian Federation. The experimental groups included cows with clinical signs (No. 1) and subclinical form (No. 2) of mastitis with positive results of studies using the KENOTEST reagent. Antibiotic sensitivity of bacterial isolates isolated from milk was determined by the cylinder method. In cows with a subclinical and clinically pronounced form of mastitis, Staphylococcus aureus, Corynebacterium spp., Streptococcus agalactiae, Bacillus spp. (β-hemolytic isolates) were isolated during bacteriological examination. The maximum effectiveness of MASTIBLOCK® DC 49 has been established in the subclinical serous-catarrhal form of mastitis, mainly against Grampositive bacteria. MASTIGARD has a wider range of antimicrobial effects, including on strains producing β-lactamase and inhibiting the action of penicillin antibiotics. The results obtained allow us to recommend the use of the complex antibiotic MASTIBLOCK® DC 49 not only during the dry season, but also during lactation to stop bacterial infection of the breast in cows, while observing the appropriate technology of milking and milk processing.
Keywords
About the Authors
S. V. EngashevRussian Federation
Engashev S.V. – Professor, Doctor of Veterinary Sciences, Academician of the Russian Academy of Sciences, Professor of the Department of Parasitology and Veterinary and Sanitary Expertise
M. D. Novak
Russian Federation
Novak M.D. – Professor, Doctor of Biology, Professor of the Department of Epidemiology
A. I. Novak
Russian Federation
Novak A.I. – Associate Professor, Doctor of Biology, Professor of the Department of Microbiology
O. V. Evdokimova
Russian Federation
Evdokimova O.V. – Associate Professor, Candidate of Medical Sciences, Head of the Department of Microbiology
E. S. Engasheva
Russian Federation
Engasheva E. S. – Doctor of Biology, Scientific sotr. lab. Pharmacology and Toxicology
References
1. Semivolos, A. M. Recommendations for the diagnosis, therapy and prevention of mastitis in cows / A. M. Semivolos, V. S. Avdeenko, V. G. Gavrish. – Saratov, 2009: 71. URL: https://www.elibrary.ru/item.asp?id=26269916 (In Russ.)
2. Petrov, R. V. Immunology / R. V. Petrov. – Moscow: Publishing house "Medicine", 1987: 414. (In Russ.)
3. Pogodaeva, P. S. Some aspects of the local immune response in breast tissues / P. S. Pogodaeva, L. Y. Karpenko, V. S. Ponamarev // International Bulletin of Veterinary Medicine. 2020:4:129-133. – DOI 10.17238/issn2072-2419.2020.4.129. URL: https://www.elibrary.ru/item.asp?id=44402163 (In Russ.)
4. Simonov, Yu. I. Conditions of maintenance as an etiological factor of the occurrence of diseases in dairy cows with industrial maintenance / Yu. I. Simonov, L. N., Simonova, I. V. Malyavko // Zootechnia. 2021:4:23-27. – DOI 10.25708/ZT.2021.31.46.007. URL: https://www.elibrary.ru/item.asp?id=46246405 (In Russ.)
5. Rudenko, P. A. Microbial landscape with mastitis in cows / P. A. Rudenko, A. A. Rudenko, Yu. A. Vatnikov // Bulletin of the Ulyanovsk State Agricultural Academy. 2020:2(50):172-179. – DOI 10.18286/1816-4501-2020-2-172-179. URL: https://cyberleninka.ru/article/n/mikrobnyy-peyzazh-pri-mastitah-u-korov (In Russ.)
6. Manzhurina, O. A. Monitoring of sensitivity of staphylococcus aureus isolated from milk of cows with mastitis / O. A. Manzhurina, A.M. Skogoreva, Y. S. Parkhomenko // Veterinary and sanitary aspects of quality and safety of agricultural products: Proceedings of the II-th International Conference on Veterinary and sanitary expertise. Voronezh State University. Voronezh, 2017:179-183. URL: https://www.elibrary.ru/item.asp?id=34987780 (In Russ.)
7. Ryshchanova, R. M. Antibiotic resistance and the ability to form biofilms of staphylococcus aureus isolated from cow milk of the Kostanay region of the Republic of Kazakhstan / R. M. Ryshchanova, A.M. Mendybaeva, G. B. Mukanov // Innovations and food security. 2021:3(33):29-39. – DOI 10.31677/2072-6724-2021-33-3-29-39. URL: https://www.elibrary.ru/item.asp?edn=bprfpp (In Russ.)
8. Skogoreva, A.M. Antibiotic sensitivity of mastitis pathogens in cattle breeding in the Voronezh region / A.M. Skogoreva, O. A. Manzhurina, O. V. Popova // Topical issues of veterinary medicine and animal husbandry technology: Materials of the scientific and educational-methodical conference. Voronezh State Agrarian University named after Emperor Peter. Voronezh, 2019:8:205-206. URL: https://www.elibrary.ru/tfkyvv (In Russ.)
9. Firsov, G. M. Mastitis in young pregnant cows and heifers and antibiotic resistance of its pathogens / G. M. Firsov, A. A. Ryadnov, T. A. Ryadnova, Yu. G. Firsova // Scientific substantiation of the strategy of digital development of agriculture and rural territories: materials of the National scientific and practical conference. Volgograd State University. Volgograd, 2023:331-336. URL: https://www.elibrary.ru/item.asp?edn=dlklkp (In Russ.)
10. Kalsoom, F. A model of antibiotic resistance against various Staphylococcus aureus isolates from raw milk samples / F. Kalsoom, N. H. S. Syed, J. Farzana // J. Res. Sci. 2004:15:145-151.
11. Semina, L. K. Sensitivity of the udder microflora of cows with mastitis to complex antimicrobial drugs with and without prednisone / L. K. Semina, Z. A. Skulyabina, S. V. Timoshina, N. N. Avduevskaya // Veterinary medicine of Kuban. 2019:2:8-10. – DOI 10.33861/2071-8020-2019-2-8-10 URL: http://vetkuban.com/num2_201903.html (In Russ.)
12. Cherdakova, V. O. Mastitis in cows: optimization of animal treatment regimens in the direction of reducing the level of antibiotic resistance in the microflora causing them / V. O. Cherdakova, A. S. Dimova // Issues of veterinary science and practice: Proceedings of the scientific and practical conference. Novosibirsk State University. Novosibirsk, 2022:104-106. URL: https://www.elibrary.ru/item.asp?id=48548967&pff=1 (In Russ.)
Review
For citations:
Engashev S.V., Novak M.D., Novak A.I., Evdokimova O.V., Engasheva E.S. SENSITIVITY OF BACTERIA TO ANTIBIOTICS MASTI BLOCK® DC 49, MASTIC-P, MASTERCARD WITH MASTITIS OF DRY COWS. International Journal of Veterinary Medicine. 2024;(4):161-171. (In Russ.) https://doi.org/10.52419/issn2072-2419.2024.4.161