DIAGNOSTICS OF LIVER DISEASES IN CATTLE USING CAFFEINE CLEARANCE, WITH FATTY HEPATOSIS AS AN EXAMPLE

Abstract

Hepatic elimination is a function of interactions between various processes, such as sinusoidal absorption, intracellular metabolism, canalicular (biliary) secretion and sinusoidal outflow. The aim of our study was to investigate the relationship between the clinical condition of cattle with a confirmed diagnosis of fatty hepatosis and the metabolism of sodium caffeine benzoate. The studies were conducted on a farm in the Pskov region; the object of the study was Holsteinized cows, which underwent a medical examination, according to the results of which animals with fatty hepatosis were selected. The experimental animals were injected once with a sterile solution of sodium caffeine benzoate (OOO Mosagrogen, Russia) at a dosage of 5 mg / kg (according to the instructions and previously developed methods). The control group, healthy animals, were injected with the same drug in a similar dosage. After 1, 2, 3, 4, 5, 6, 8, 10, 12, 18 and 24 hours, blood was collected from the experimental animals by venipuncture of the jugular vein, after which caffeine levels were determined by the enzyme immunoassay method using the ELISA-Caffeine test system (manufacturer Gold Standard Diagnostics, USA) on a Stat Fax 4700 strip ELISA analyzer (USA). The correlation model for diagnosing hepatobiliary pathologies based on caffeine clearance was studied by identifying correlation coefficients (Spearman and Pearson). With normal liver function, caffeine is eliminated within the expected time frame, but in the presence of pathologies, a delay ("plateau") in the elimination of the substance is observed. Based on the analysis of the data obtained, it is possible to create prognostic models that allow veterinary specialists not only to diagnose, but also to form an idea of the course of diseases.