COMPARATIVE ANALYSIS OF THE ANTAGONISTIC ACTIVITY OF LACTOBACILLI OF THE PROBIOTIC BIOCOMPLEX «AVIBIOLACT»

Currently, the current method of correction and maintenance of the balance of intestinal microflora of birds is the use of probiotics - biopreparations created on the basis of live microbial cultures. Of particular interest for the prevention of gastrointestinal diseases of birds are probiotics based on lactobacilli with high antagonistic ability, which determines their practical application in poultry farming. The introduction of probiotics into the diet of birds from the first days of life stabilizes the microflora of the gastrointestinal tract and improves physiological processes, which contributes to increased safety and increased productivity of birds. The article presents data from a comparative study of the antagonistic activity of the probiotic biocomplex "AVIBIOLAKT" and individual cultures of lactobacilli included in this biopreparation - Lactobacillus acidophilus, Lactococcus lactis, Lactococcus cremoris. Isolates of pathogenic and opportunistic microorganisms from the collection of the Museum of Working Cultures of the Bacteriology Department of the All-Russian Research Institute of Vitiligo and Infectious Diseases were used as test cultures: Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Pseudomonas aeruginosa. The present studies have confirmed the antagonistic efficiency of the biocomplex "AVIBIOLAKT" in relation to opportunistic and pathogenic test microorganisms. The probiotic biocomplex "AVIBIOLAKT" had the highest antagonistic activity in relation to test microorganisms. It formed a growth inhibition zone from 16.4 to 19.6 mm depending on the test strain. Thus, the growth inhibition zone of Staphylococcus aureus was 19.6 mm, Escherichia coli and Salmonella enteritidis - 19.3 and 18.8 mm, respectively, and Pseudomonas aeruginosa - 16.1 mm. It was established that the level of antimicrobial activity against test microorganisms - Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Pseudomonas aeruginosa. was expressed to a greater extent in the biocomplex "AVIBIOLAKT". In individual cultures of lactobacilli of the studied probiotic biocomplex, the antagonistic ability was less pronounced. Lactobacillus acidophilus had the greatest antagonistic activity among individual cultures of lactobacilli.

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