PREVENTION OF METABOLIC DISORDERS IN RATS DURING STRESS MODELING

Abstract

Stress in animals poses a serious problem that affects their health, productivity, and overall well-being. In modern livestock farming, it is extremely important to develop methods that help minimize stress and its consequences. One such approach is the use of organic acids, which have a positive impact on metabolism and adaptive processes in animals. The aim of this study was to investigate the effects of the organic-mineral supplement Klim on metabolic processes under conditions of cold stress. For this purpose, a study was conducted with laboratory rats, which were divided into three groups: intact, control (subjected to cold stress), and experimental (receiving the Klim supplement under stress conditions). Cold stress was modeled by placing the animals in a freezer at a temperature of -18°C for 4 hours. The results showed that stress significantly impaired protein metabolism indicators and activated catabolic processes in the control group, as evidenced by increased levels of total protein and urea, as well as decreased alkaline phosphatase activity. In the experimental group receiving the Klim supplement, there were no significant changes in protein metabolism parameters, indicating its effectiveness in reducing the negative effects of stress. The study confirmed that the Klim supplement contributed to the improvement of the overall condition of the animals and their adaptation to stress conditions, highlighting the potential for using organic additives in livestock farming to enhance health and productivity. The results indicate the need for further research into the application of various additives to minimize stress and improve the well-being of animals in the agricultural sector.