INDUCING OF CHRONIC KIDNEY DISEASE IN RATS USING THE MODIFIED 5/6 NEPHRECTOMY
https://doi.org/10.52419/issn2072-2419.2024.4.364
Abstract
Chronic kidney disease (CKD) is a wide spread human illness defined as long-lasting abnormalities of kidney structure or function due to fibrosis and destruction of the normal architecture of the kidney. Pathophysiology of this process is not completely understood; thus, development of prophylactics, early detection and therapy of initial stage are complicated. Therefore, inducing of CKD in local research centers is a relevant objective for Russian pharmacology. 5/6 nephrectomy in rats is recommended by many scientists as “gold standard'” for this pathology. In this study CKD was successfully induced in Wistar rats using 5/6 nephrectomy, modified to meet the requirements of a large preclinical center. Two animal groups (control and pathology) underwent a complex of clinical diagnostic tests and pathological examination, so the CKD criteria could be specified. Elevated blood pressure and decreased glomerular filtration rate enabled assessment of the disease progress in experimental animals. At the end of the study these symptoms were partly reversible revealing prominent adaptability of rat kidney. In order to confirm pathology formation, we performed histological (hematoxylin-eosin, Masson's trichrome) and immonohistochemical (CD68, Caspase3) staining of kidney sections. During pathomorphological study we found alterations specific for CKD: apoptosis of tubule epithelium and fibrosis area crowded by CD68+ macrophages. Thus, we identified antemortem and postmortem criteria of CKD induced by modified 5/6 nephrectomy in rats in Russian research center.
About the Authors
A. A. Matichina
RMC «HOME OF PHARMACY»
Russian Federation
Russian Federation
Matichina A.A. – Research Scientist of the Department of Specific Pharmacology and Pharmacodynamics
V. D. Karanina
RMC «HOME OF PHARMACY»
Russian Federation
Russian Federation
Karanina V.D. – Research Scientist of the Department of Specific Pharmacology and Pharmacodynamics
Zh. Yu. Ustenko
RMC «HOME OF PHARMACY»
Russian Federation
Russian Federation
Ustenko Zh.Yu. – Candidate of Sciences in Veterinary, Deputy Head of Department of Histology and Pathomorphology
М. V. Miroshnikov
RMC «HOME OF PHARMACY»
Russian Federation
Russian Federation
Miroshnikov М.V. – Candidate of Sciences in Medicine, Head of the Laboratory Diagnostics Department
A. A. Matichin
RMC «HOME OF PHARMACY»
Russian Federation
Russian Federation
Matichin A.A. – Head of the Department of Specific Pharmacology and Pharmacodynamics
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Review
For citations:
Matichina A.A., Karanina V.D., Ustenko Zh.Yu., Miroshnikov М.V., Matichin A.A. INDUCING OF CHRONIC KIDNEY DISEASE IN RATS USING THE MODIFIED 5/6 NEPHRECTOMY. International Journal of Veterinary Medicine. 2024;(4):364-373. (In Russ.) https://doi.org/10.52419/issn2072-2419.2024.4.364
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