PATHOGENETIC ASPECTS OF THE USE OF CONDITIONED MEDIUM FROM STROMAL STEM CELLS IN EQUINE ASTHMA SYNDROME

Abstract

Modern medical and veterinary practice is increasingly focused on the use of organsparing and genetically molecular technologies that help restore lost functions. In this context, cellular technologies, including the creation of recombinant chemokines and monoclonal antibodies, play an important role in the targeted therapy of serious diseases. Cellular regenerative medicine finds its application in the healing of skin and muscle structures, especially in horses. In this paper, the possibility of using the secretome of mesenchymal stromal cells (MSCs) for the treatment of inflammatory diseases of the bronchopulmonary system in horses suffering from asthma is considered. The study was conducted on the basis of a retrospective analysis of observations of 91 horses that had signs of asthma (EAS). 70 of these horses were treated with the secret of MSC (the drug Reparin helper). Both mares and geldings aged 10-21 years took part in short-term observations. The secret was administered intrabronchially, inhaled and intravenously. The effectiveness of the treatment was assessed by changes in the clinical condition and possible adverse reactions. Analysis of the results of short-term observations showed that the majority of patients treated with secret MSC had no negative reactions. The clinical efficacy of the drug varied depending on the method of administration: the intratracheal method showed no improvement, while inhalation and intravenous administration led to positive results. Horses with severe asthma had a high degree of exercise tolerance against the background of the drug, which confirms its effectiveness. Horses that received the secret of MSC for a long time showed stable remission for 6-12 months. Among the 70 cases of the use of the MSC secretome, the results showed the most positive effects in horses with severe asthma. More than 20% of them successfully received additional therapy for two years, demonstrating long periods of remission. The study confirms the potential of using the MSC secretome in veterinary practice, and the data obtained may be useful for humane medicine.