THE USE OF TARGETED SEQUENCING FOR GENOTYPING SHEEP OF THE MANYCH MERINO BREED

The main use of genetic data is to assess the reliability of origin, since errors in pedigrees negatively affect the rate of genetic growth. Initially, the assessment of the pedigree of breeding livestock was carried out using various DNA technologies: from blood groups, then using microsatellite markers, however, now the possibility of using single nucleotide polymorphisms (SNP) in practice is being increasingly studied, due to their ability to increase the accuracy of genetic research. One of such genotyping systems is AgriSeq panels (targeted sequencing technology), which allow analyzing specific regions of the genome (loci) associated with a variety of traits in animals. The purpose of the work: to study the effectiveness of identifying and prevalence of loci from the proposed set of SNPs in the examination of new generations of sheep of the Manych Merino breed. The material for the study was data from targeted sequencing of the genomes of Russian sheep breeds according to the formed set of loci in order to identify singlenucleotide polymorphisms. The object of the study was sheep born in 2021 at the age of 12 months (DNA sampling was carried out in 2022) of the Manych merino breed. The proposed locus panel, modified after validation on the next generation of animals, contains 389 substitutions suitable for genotyping by sequencing and 242 polymorphisms associated with animal meat productivity. The indicators of the frequency of occurrence of substitutions in the group of selected animals obtained as a result of research allow us to conclude that the polymorphisms, we have selected are located in loci that are not subject to significant rearrangements for several generations and can be informative for quite a long time. The analysis allows us to conclude that after modification of the set of loci, the panel for genotyping by sequencing in the Manych merino showed high efficiency in identifying all variants of genotypes.

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