PROSPECTS FOR USING COMPONENT MESENCHYMAL STEM CELLS AS A WAY TO IMPROVE THE QUALITY OF BULLS’ SPERM

Abstract

The aim of this study was to investigate the effect of mesenchymal stem cell derivative (lysate) from adipose tissue (AT) and bone marrow (BM) of bulls on the quality of native sperm. A total of 10 samples were taken. Each sample was divided into aliquots (after centrifugation (7 min 600 rpm) and removal of seminal plasma) according to the following groups: control (sperm 7x10¹⁰ cells/ml + PBS buffer 100 μl), the first experimental (sperm 7x10¹⁰ cells/ml + BM MSC lysate 2x10⁸ cells/ml), the second experimental (sperm 7x10¹⁰ cells/ml + AT MSC lysate 2x10⁸ cells/ml).A four-stage assessment of sperm quality was carried out: 0, 2 and 4 hours of incubation at a temperature of 37℃.According to the results of the studies, a reliable decrease in the number of normal spermatozoa was recorded when assessing 4-hour sperm incubation.At the same time, the difference in values in the control and first experimental groups was 1.3 times (p < 0.05 for both groups), in the second experimental - 1.4 times (p < 0.05) compared with the data of 0 hours of incubation.The highest number of morphologically normal spermatozoa was recorded in the first experimental group, when incubating spermatozoa with bone marrow MSC secretion products.A statistically significant decrease in the number of progressively moving spermatozoa was obtained after 2 and 4 hours of incubation compared with the data obtained when assessing motility at 0 hours of incubation.Thus, the difference in values in the control group was 1.4 (2 and 4 hours, p<0.01), in the first experimental group - 1.1 (p<0.05) and 1.4 (p<0.01), in the second experimental group - 1.2 (p<0.05) and 1.6 (p<0.05) times 2 and 4 hours, respectively.The highest number of progressively moving spermatozoa was recorded during the interaction of bovine germ cells with the MSC lysate during two hours of incubation, obtained from the bone marrow and amounted to 74.75±2.39%.