STALLION SPERM SURVIVAL IN VARIOUS EXTENDERS WHEN COOLING AT 4-5°C
https://doi.org/10.52419/issn2072-2419.2024.4.539
Abstract
Most equine artificial insemination is now carried out using chilled semen, as its fertilizing capacity is higher than cryopreserved semen. However, the possibility of long-term storage of chilled sperm is limited. The use of extenders allows to prolong the preservation of the biological value of chilled sperm. In this study, the survival rate and morphology of chilled stallion sperm were assessed using different extenders. Four extenders were used: LHСZh, recommended by the All-Russian Research Institute of Horse Breeding, Kenny, widely used abroad, extender for cooling sperm of the All -Russian Research Institute of Farm Animal Genetics and Breeding, and an experimental extender based on TRIS and fructose. A reliable difference was observed in the total and progressive motility of sperm diluted with the experimental and other media during storage for 24, 48 (P˂0.05) and 72 hours (P˂0.01). After storage for 72 hours, sperm diluted with the experimental extender had an average total motility over 50% and a progressive motility 48.2±3.67%, which makes it possible to use it for artificial insemination. Evaluation of sperm morphology showed good preservation of cells during 72-hour storage at 4-5 0C when using all extenders. The results of this study showed preservation of sperm quality necessary for artificial insemination for 48 hours, and when diluted with the experimental extender for 72 hours.
Supporting agencies: The work was carried out with the financial support of the Ministry of Education and Science, project No. 124020200127-7
About the Author
E. V. Nikitkina
Russian Research Institute of Farm Animal Genetics and Breeding — Branch of the L.K. Ernst Federal Research Center for Animal Husbandry
Russian Federation
Russian Federation
Nikitkina E.V. – Ph.D. biological sciences, laboratory of developmental biology
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Review
For citations:
Nikitkina E.V. STALLION SPERM SURVIVAL IN VARIOUS EXTENDERS WHEN COOLING AT 4-5°C. International Journal of Veterinary Medicine. 2024;(4):539-546. (In Russ.) https://doi.org/10.52419/issn2072-2419.2024.4.539
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