The effect of an infectious dose of Escherichia coli on the effectiveness of levofloxacin in experimental colibacteriosis of chickens

The aim of the present research was to study the impact of infective dose on effectiveness of levofloxacin in Antibiotic therapy solves the problems of eliminating colibacillosis, and properly constructed treatment regimens prevent the spread of resistant strains of pathogens among humans, farm animals and birds. The aim of this work was to study the effect of the infective dose of Escherichia coli on the effectiveness of levofloxacin in experimental colibacillosis of chickens. For the experiment, 375 chickens were used, which were divided into three groups of five subgroups (25 heads) in each. The birds of the first group were infected intraperitoneally with a suspension from a daily culture of E. coli at a concentration of 150 million microbial bodies / 0.5 ml (1 DLM - minimum lethal dose). Birds of the second and third groups were infected in a similar manner, with the minimum lethal dose increased to 2 DLM (300 million microbial bodies/0.5 ml of suspension) and 3 DLM (450 million microbial bodies/0.5 ml of suspension), respectively. The chickens were observed for 15 days. The efficacy of levofloxacin administered in various concentrations with drinking water was assessed by comparative analysis of the number of surviving and dead birds in the experimental and control groups. When reproducing experimental colibacillosis in chickens with an infectious dose of 1 DLM, a high therapeutic effect (100%) was achieved in the groups of birds to which the drug was administered in concentrations of 200 and 300 mg/l of water. In the groups of chickens where the infectious dose was increased to 2 DLM, the efficacy of treatment decreased to 80-84%. In groups of chickens where the challenge dose was increased to 3 DLM, treatment efficacy decreased to 64-80%.

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