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The main biochemical parameters of the blood of Duroc pigs in comparison with their three-breed hybrids in the dynamics of fattening

https://doi.org/10.52419/issn2072-2419.2025.1.250

Abstract

In the last decade, significant progress has been achieved in pork production and Russian pig farming in general. However, it is necessary to intensify work on establishing standards for the main biochemical (BC) parameters of the blood of commercial pig hybrids, which was the main goal of the work. The studies were conducted on boars of the following groups: 1) Duroc breed on the 60-65th day of fattening (n=12), 2) F2 Duroc˟Landrace˟Large White hybrids on the 60-65th day of fattening (n=19), 3) Duroc breed on the 100-110th day of fattening (n=18) and 4) F2 Duroc˟Landrace˟Large White hybrids on the 100-110th day of fattening (n=27). Blood samples were taken from the ear vein into vacuum tubes with a blood coagulation activator for biochemical coagulation analysis and centrifuged (15 min at 3000 rpm). Biochemical coagulation analysis was performed on a ChemWell analyzer (Awareness Technology, USA). Amino acid concentrations in blood serum were determined by ion-exchange chromatography on a HPLC LC-20 Prominence (Shimadzu, Japan) with a proprietary column and a module for post-column derivatization with ninhydrin ARM-1000 (Sevko&Co, Russia). Samples for analysis were prepared according to GOST 32195-2013. It is positive that the main protein indices of blood in Duroc pigs and their F2 hybrids in different age groups are quite stable, as evidenced by low and moderate variation coefficients (CV<20%). It is shown that with an increase in the fattening period, the tendencies to intensify all types of metabolism increase, as evidenced by numerous correlations between the biochemical and amino acid parameters of blood of boars of groups 3 and 4 at the end of fattening (100-110 days) compared to groups 1 and 2 (a period of 60-65 days). The authors believe that establishing relationships between the biochemical parameters of pig blood, on the one hand, and their breed characteristics and fattening duration, on the other hand, is of great fundamental and practical importance.

About the Authors

S. Yu. Zaitsev
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation

Zaitsev S. Yu. – Doctor of Chemical Sciences, Doctor of Biological Sciences, leading researcher, Head of the Analytical Biochemistry Group



N. S. Kolesnik
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation

Kolesnik N. S. – Junior Researcher, Department of Physiology and Biochemistry of farm animals



A. A. Savina
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation

Savina A. A. – Junior Researcher, of the Analytical Biochemistry Group



O. A. Voronina
Federal Research Center for Animal Husbandry named after Academy Member L.K. Ernst
Russian Federation

Voronina О. А. – Candidate of Biological Sciences, Senior Researcher of the Analytical Biochemistry Group



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For citations:


Zaitsev S.Yu., Kolesnik N.S., Savina A.A., Voronina O.A. The main biochemical parameters of the blood of Duroc pigs in comparison with their three-breed hybrids in the dynamics of fattening. International Journal of Veterinary Medicine. 2025;(1):250-264. (In Russ.) https://doi.org/10.52419/issn2072-2419.2025.1.250

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ISSN 2072-2419 (Print)