Cytokine profile in newborn calves after antenatal and intranatal hypoxia
https://doi.org/10.52419/issn2072-2419.2025.1.326
Abstract
The aim of the study was to investigate the cytokine profile of newborn calves that had undergone antenatal and intranatal hypoxia. Three groups of calves took part in the study: healthy (n = 8); those that had undergone antenatal hypoxia (n = 14); those that had undergone intranatal asphyxia (n = 12). Three days after birth, blood samples were taken from these animals to determine the concentration of interleukin-1β (IL-1β), interleukin-10 (IL-10) and interferon-γ (IFN-γ) using the enzyme immunoassay. It was found that in calves with antenatal and intranatal hypoxia, the cytokine profile is characterized by an increased level of IL-1β by 14.9-84.4% (P 0.05) and a decreased level of IL-10 produced by type II T-lymphocytes by 18.3-19.8% (P 0.05). At the same time, the amount of INF-γ produced by type I Tlymphocytes in calves with antenatal hypoxia does not change significantly, whereas in calves with intranatal hypoxia this indicator increases by 14.7% (P 0.05). When calculating IL-1β/IL-10, it was noted that in animals that have undergone antenatal and intranatal hypoxia, this indicator significantly exceeds the value of healthy animals. Thus, for the former it is higher by 43.5% (P 0.05), and for the latter by 2.3 times (P 0.05). In turn, the calculation of the INF-γ/IL-10 ratio shows that in calves that have experienced antenatal and intranatal hypoxia, this indicator is higher by 27.2 and 40.8% (P 0.05), respectively. Thus, the study showed that in newborn calves that have experienced hypoxic conditions, the activity of T-cell immunity and proinflammatory cytokine profile prevail, which reflects the severity of the pathological effect.
About the Authors
M. S. Zhukov
All-Russian Scientific Research Veterinary Institute of Pathology, Pharmacology
and Therapy
Russian Federation
Russian Federation
Zhukov M.S. – cand. veterinarian. sciences, art. sci. sotr. Department of Experimental Pharma-cology and Functioning of Living Systems
P. A. Parshin
All-Russian Scientific Research Veterinary Institute of Pathology, Pharmacology
and Therapy
Russian Federation
Russian Federation
Parshin P.A. – Doctor of Veterinary Sciences, Professor, Director
Yu. N. Alekhin
All-Russian Scientific Research Veterinary Institute of Pathology, Pharmacology
and Therapy
Russian Federation
Russian Federation
Alekhin Yu.N. – Doctor of Veterinary Sciences, Chief Scientific Officer. sotr. Department of Experimental Therapy
I. T. Shaposhnikov
All-Russian Scientific Research Veterinary Institute of Pathology, Pharmacology
and Therapy
Russian Federation
Russian Federation
Shaposhnikov I.T. – Doctor of Biological Sciences, Chief Scientific Officer. sotr. Department of Experimental Pharma-cology and Functioning of Living Systems
Yu. N. Brigadirov
All-Russian Scientific Research Veterinary Institute of Pathology, Pharmacology
and Therapy
Russian Federation
Russian Federation
Brigadirov Yu.N. – Doctor of Veterinary Sciences, Chief Scientific Officer. sotr. Department of Experimental Pharmacology and Function-ing of Living Systems
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Review
For citations:
Zhukov M.S., Parshin P.A., Alekhin Yu.N., Shaposhnikov I.T., Brigadirov Yu.N. Cytokine profile in newborn calves after antenatal and intranatal hypoxia. International Journal of Veterinary Medicine. 2025;(1):326-332. (In Russ.) https://doi.org/10.52419/issn2072-2419.2025.1.326
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