Polymorphism of the IGF-1 gene in the livestock of Holstein cows breeds and breeding bulls

Insulin-like growth factor-1 (IGF-1), secreted in the liver, is one of the major elements of the somatotropic axis, is involved in numerous physiological processes, and plays a role in the control of lactation, mammary gland development, and the formation of important performance traits such as growth and reproductive traits. The associations of IGF-1 gene polymorphism (T → C 512) have been studied and evaluated with economically useful traits in different breeds of dairy and beef cattle. The study carried out on the population of Holstein cows and Holstein and Holsteinized bullssires allowed to establish that the identified populations are polymorphic, the animals are represented by three genotypes of IGF-1 gene. The frequency of occurrence of IGF-1 gene genotypes in the cow population amounted to: AA - 24.7 %, AB and BB - 55.6 and 27.3 %, and in bulls-sires: AA – 31.3 %, AB – 49.3 % and BB – 19.4 %. In both cases, the predominance of the “normal” allele over the “mutant” allele was observed, with the A allele prevailing over the B allele to a greater extent in the group of breeding bulls. The analysis of literature data indicates that the frequency of alleles (A and B) and genotypes (AA, AB and BB) in populations shows variation depending on breed, direction of productivity and habitat of cattle.

1. Belaya E.V., Mikhailova M.E. SnaBIPolymorphism of the gene for insulin-like growth factor (bIGF-I) in cattle // Molecular and applied genetics. 2008. 8:153-159.

2. Vafin R.R., Tulkin S.V., Zagidullin L.R., Muratova A.V., Akhmetov T.M., Zinnatova F.F., Ravilov R. Polymorphism of genes of growth hormone-releasing hormone and insulin-like growth factor in bulls of the Republic of Tatarstan // Achievements of science and technology of agroindustrial complex. 2017. 31(4):75-78.

3. Gaynutdinova E.R., Safina N.Yu., Shakirov Sh.K. Compatibility of dairy productivity and reproductive capacity of Holstein breed cows // Vestnik of Kazan State Agrarian University. 2020. T 15. Vol 2(58):5-9.

4. Zagidullin L.R., Gilemhanov I.Y., Akhmetov T.M., Shaidullin R.R., Tyulkin S.V. Milk productivity and milk quality of cows with different genotypes by the IGF1 gene // Bulletin of Michurinsk State Agrarian University. 2021. 4 (67):135-139

5. Safina N.Yu., Gilemhanov I.Y., Zinnatova F.F., Shakirov Sh.K. Characteristic of milk productivity of cows-heifers with different genotypes of somatotropin (GH) // Vestnik of Kazan State Agrarian University. 2019. T 14. Vol 3(54): 58-61. DOI:10.12737/article_5db9535ed384a3.87060395

6. Akis I., Oztabak K., Gonulalp I., Mengi A., Un C. IGF-1 and IGF-1R gene polymorphisms in East Anatolian Red and South Anatolian Red cattle breeds // Russian Journal of Genetics. 2010. 46(4):439-442. DOI: 10.1134/s1022795410040083

7. Anggraeni A. Talib C., Asmarasari S.A., Herawati T., Andreas E. Polimerfisme genetik dari gen IGF1, GH dan OPN pada persilangan sapi PO berdasarkan tipe kelahiran di Jawa Tengah // JITV. 2017. 22(4):165-172. DOI: 10.14334/jitv.v22i4.1625

8. Arslan K., Taheri S., Şener E.F., Akyüz B., Akçay A., Özkul Y., İşcan K.M. Investigation of the promoter polymorphisms of the growth hormone (GH1), growth hormone receptor (GHR), insulin-like growth factor (IGF-I), and prolactin (PRL) genes and the correlation between gene expression and milk yields in Holstein cattle raised in Central Anatolia // Turkish Journal of Veterinary & Animal Sciences: 2016. 40(5):13. DOI: 10.3906/vet-1510-66

9. De Riek J., Calsyn E., Everaert I., Van Bockstaele E., De Loose M. // AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties // Theor. Appl. Genet. 2001. 103:1254-1265. DOI: 10.1007/s001220100710

10. Çobanoğlu, Ö. and Ardiçli S. Genetic variation at the OLR1, ANXA9, MYF5, LTF, IGF1, LGB, CSN3, PIT1, MBL1, CACNA2D1, and ABCG2 loci in Turkish Grey Steppe, Anatolian Black, and East Anatolian Red cattle // Turkish Journal of Veterinary & Animal Sciences. 2022. 46(3):494-504. DOI: 10.55730/1300-0128.4198

11. De la Rosa Reyna X.F., Montoya H.M., Castrellón V.V., Rincón A.M.S., Bracamonte M.P., Vera W.A. Polymorphisms in the IGF1 gene and their effect on growth traits in Mexican beef cattle // Genetics and Molecular Research. 2010. 9(2):875-883

12. Ge W., Davis M.E., Hines H.C., Irvin K.M., and Simmen R.C.M. Association of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle // J. Anim. Sci. 2001. 79:1757–1762.

13. Han Y., Wang S., Wang Y., Zeng S. IGF -1 inhibits apoptosis of porcine primary granulosa cell by targeting degradation of BimEL // Int. J. Mol. Sci. 2019. 20 (21):5356. DOI: 10.3390/ijms20215356

14. Hartanto S., Budiyanto A., Widayanti R., Setyawan E.M.N., and Prasetya I.D. Characterization of polymorphisms in the folliclestimulating hormone receptor and insulinlike growth factor-1 genes and their association with fertility traits in Jawa-Brebes cows // Veterinary World. 2023. 16(4): 711–716. DOI: 10.14202/vetworld.2023.711-716

15. Hax L.T., Schneider A., Jacometo C.B., Mattei P., da Silva T.C., Farina G., Corrêa M.N. Association between polymorphisms in somatotropic axis genes and fertility of Holstein dairy cows // Theriogenology. 2017. 88:67–72. DOI: 10.1016/j.theriogenology.2016.03.044

16. Mehmannavaz Y., Amirinia C., Bonyadi M., Vaez Torshizi R. Association of IGF-1 gene polymorphism with milk production traits and paternal genetic trends in Iranian Holstein bulls // African Journal of Microbiology Research. 2010. 4(1):110-114.

17. Mullen M.P. Lynch C.O., Waters S.M., Howard D.J., O'Boyle P., Kenny D.A., Buckley F., Horan B., Diskin M.G. Single nucleotide polymorphisms in the growth hormone and insulin-like growth factor-1 genes are associated with milk production, body condition score and fertility traits in dairy cows // GMR. 2011. 10:1819-1830.

18. Nicolini P., Carriquiry M., Meikle A. A polymorphism in the insulin-like growth factor 1 gene is associated with postpartum resumption of ovarian cyclicity in Holstein-Friesian cows under grazing conditions // Acta Veterinaria Scandinavica. 2013. 55 (1):11. DOI: 10.1186/1751-0147-55-11

19. Siadkowska E., Zwierzchowski L., Oprządek J., Strzałkowska N., Bagnicka E., Krzyżewskiet J. Effect of polymorphism in IGF-1 gene on production traits in Polish Holstein-Friesian cattle // Animal Science Papers and Reports. 2006. 24(3):225-237

20. Silveira P.A.S., Butler W.R., da Silva T.C., Corrêa M.N., C.C. Barros, Schneider A. Association of polymorphisms in the IGF -I, GHR and STAT5A genes with serum IGF -I concentration and reproductive performance of Holstein dairy cows // Animal Reproduction Science. 2019. 211:10620. DOI: 10.1016/j.anireprosci.2019.106206

21. Szewczuk M., Bajurna M., Zych S., Kruszyński W. Association of insulin-like growth factor I gene polymorphisms (IGF1/TasI and IGF1/SnaBI) with the growth and subsequent milk yield of Polish HolsteinFriesian heifers // Czech J. Anim. Sci. 2013. 58 (9):404-411

22. Ulyanov V.A., Kubekova B.Z., Beishova I.S., Belaya A.V., Papusha N.V. Preferred and undesirable genotypes of bGH and bIGF -1 genes for the milk yield and quality of black-and-white breed // Veterinary World. 2021. 14(5):1202-1209. DOI: 10.14202/vetworld.2021.1202-1209

23. Wang, Y., Price, S. E., and Jiang, H. Cloning and characterization of the bovine class 1 and class 2 insulin-like growth factor -I mRNAs // Domest. Anim. Endocrinol. 2003. 25:315–328. DOI: 10.1016/j.domaniend.2003.06.001

24. Wasielewska, M. and Szatkowska I. Possible relationship between IGF-1/SnaBI genotypes and milk yield of Holstein-Friesian cows // Animal Science and Genetics. 2019. 15(4):35-41. DOI: 10.5604/01.3001.0013.6365

25. Yazdanpanah A.A., Roshanfekr H., Mirzadeh K., Mamouei M., Khederzadeh S. Polymorphism of insulin-like growth factor 1 gene in Najdi cattle populations // American Journal of Biochemistry and Biotechnology. 2013. 9:300-306. DOI:10.3844/ajbbsp.2013.300.306

26. Yurnalis S., Arnim, Putra D.E. Polymorphism of Insulin-like Growth Factor 1 Gene (IGF1/TasI, IGF1/SnaBI, IGF1/RsaI) and the Association with Daily Gain of Pesisir Cattle Local Breed from West Sumatera, Indonesia // Pak J Biol Sci. 2017. 20(4):210-216. DOI: 10.3923/pjbs.2017.210.216.