The effect of the drug "Prostimul" on the mitotic activity of jejunum cells in piglets with perinatal hypotrophy

Abstract

The article describes the results of a study aimed at studying the mitotic activity of jejunum cells in healthy piglets and piglets with perinatal hypotrophy, as well as evaluating the effect of the drug “Prostimul” on the regenerative processes in the intestine in this pathology. Newborn piglets were selected for the study and the following groups were formed: healthy piglets (the first group) and piglets with perinatal hypotrophy, which were further divided into two groups: The second group - piglets with perinatal hypotrophy (n=20), which per os in addition to the main diet were given cow's milk in a dosage of 2.5 ml per head for 3 days (scheme used in the farm) and the third group - piglets with perinatal hypotrophy (n=20), which were intramuscularly injected with the drug “Prostimul” twice, on the first and third days of life at a dosage of 0.1 ml/ kg body weight. Immunohistochemical study with Ki-67 marker was carried out in the jejunum tissue of piglets before milk intake, as well as on 7, 14, 21 days. It was found that perinatal hypotrophy is characterized by a decrease in the intensity of cell proliferation processes. The dynamics of changes in Ki-67 expression indices indicates a gradual increase in the proliferative activity of cells in the jejunum with age in all experimental groups, which corresponds to the normal postnatal development of the organ, but the rate of these changes was most pronounced in the group of hypotrophic piglets receiving the drug “Prostimul”, which reduced the lag behind the healthy animals.