Determination of the bactericidal activity of the Theotropin P+ disinfectant against Brucella abortus 54M

The success of disinfection measures in the country depends on the availability of effective drugs to the veterinary industry. For this purpose, new drugs are being researched and tested. According to international regulations, they must be safe, highly effective and comply with generally accepted standards. In the article, the authors present research data aimed at determining the bactericidal activity of the Theotropin P+ disinfectant against the causative agent of brucellosis using the suspension method and the method of neutralizing contaminated surfaces of test objects (rubber, wood, galvanized steel, concrete) that mimic livestock facilities. The reference strain B was used in the work. abortus 54M (test - culture), which was previously studied for its basic properties (tinctorial-morphological and cultural-morphological), cultivated on mowed liveropen-hearth agar with Hottinger's digest (PMHA) and liver-open-hearth broth with Hottinger's digest (PMHB) and the disinfectant "Theotropin P+" in the following concentrations 0.5%; 1%; 2%; 5%; 10% and exposures on test objects, in the first case 10; 30; 60; 120; 180 minutes, in the second – 1; 3; 24 hours. With positive (test culture) and negative (without test culture) controls. Studies have established that the Theotropin P+ product, being a new generation disinfectant, has bactericidal activity against the reference strain B. abortus 54M, the main properties of which are characteristic of this pathogen, ensuring its complete inactivation depending on the type of material of the surfaces to be disinfected. The data obtained allow us to recommend this product as a disinfectant in veterinary practice for disinfection of animal husbandry, infectious foci, transport infrastructure, warehouses, meat and dairy industries, privately and publicly owned, etc.

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